umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
PROPERTIES OF SUBMITOCHONDRIAL PARTICLES FROM PLANT-MITOCHONDRIA - GENERATION, SURFACE CHARACTERISTICS AND NAD(P)H OXIDATION
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0001-5900-7395
1991 (engelsk)Inngår i: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 78, nr 2, s. 177-183Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Purified mitochondria isolated from potato (Solunum tuberosum L. cv. Bintje) tuber, Jerusalem artichoke (Helianthus tuberosus L.) tuber and rat liver were disrupted at different pH and different EDTA and MgCl2 concentrations either by French Press treatment or by sonication. The submitochondrial particles (SMP) were isolated by differential centrifugation and polarity estimated by the latency of cytochrome c oxidase (CCO) activity. The SMP were 5-95% inside-out depending on the conditions, and the disruption method was more important than the composition of the disruption medium in determining the polarity. At pH 6 and 7 and high-salt conditions sonication yielded SMP of the same polarity (82-91% inside-out) whereas French Press treatment in a low-salt buffer + EDTA gave more inside-out SMP at pH 6 than at pH 7. The inside-out vesicles were able to build up a membrane potential in the presence of respiratory substrates (as tested with the anionic dye, oxonol VI) whereas no membrane potantial could be detected with the right-side-out vesicles (as tested with cationic dyes, and optical dye, safranine O, and a fluorescent dye, rhodamine 123) under similar conditions. Binding of Concanavalin A indicated that both the inner and outer surface of the inner membrane have exposed glycoproteins and/or glycolipids. Both right-side-out and inside-out SMP oxidized NADH, NADPH and succinate with good rates but there were clear differences in both donor and acceptor specificity between the outer and inner surface of the inner mitochondrial membrane. NADH oxidation by inside-out SMP was Ca2+-independent and rotenone-inhibited whereas NADPH oxidation by the inside-out SMP was Ca2+-dependent and relatively unaffected by rotenone.

sted, utgiver, år, opplag, sider
1991. Vol. 78, nr 2, s. 177-183
Identifikatorer
URN: urn:nbn:se:umu:diva-46917DOI: 10.1016/0168-9452(91)90196-FISI: A1991GJ17900005OAI: oai:DiVA.org:umu-46917DiVA, id: diva2:447232
Tilgjengelig fra: 2011-10-11 Laget: 2011-09-16 Sist oppdatert: 2018-06-08

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekst

Personposter BETA

Gardeström, Per

Søk i DiVA

Av forfatter/redaktør
Gardeström, Per
Av organisasjonen
I samme tidsskrift
Plant Science

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 27 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf