Umeå University's logo

umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
An in vitro Study of Drug-induced Degranulation of Human HMC-1 Mast Cells and Rat RBL-2H3 Cells
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandläkarutbildning.
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandläkarutbildning.
2014 (engelsk)Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
Abstract [en]

Mast cells and basophils participate in biological responses such as inflammation and allergic reactions and contain potent mediators such as histamine and cytokines. Some drugs, for example opioids, are known to trigger mast cell degranulation with the release of immunogenic mediators that may cause severe side effects.

The aim of this study was to investigate if the human mast cell line HMC-1 is a useful model to study codeine-induced degranulation in vitro. The rat basophilic leukemia RBL-2H3 cell line was used as a reference. Time- and concentration-dependent effects of codeine, compound 48/80, and A23187 on the degranulation of HMC-1 cells and RBL-2H3 cells were investigated by measuring extracellular levels of β-hexosaminidase and [3H]serotonin.

Neither codeine nor compound 48/80 produced any significant basophil or mast cell degranulation, whereas the calcium ionophore A23187 triggered a degranulation of both cell lines.

In conclusion, none of the cell lines are useful as relevant, robust and reproducible in vitro models to study drug-induced mast cell degranulation.

sted, utgiver, år, opplag, sider
2014.
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-97850OAI: oai:DiVA.org:umu-97850DiVA, id: diva2:777071
Utdanningsprogram
Dentistry Programme
Veileder
Examiner
Tilgjengelig fra: 2015-03-11 Laget: 2015-01-08 Sist oppdatert: 2015-03-11bibliografisk kontrollert

Open Access i DiVA

fulltext(466 kB)1424 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 466 kBChecksum SHA-512
6209d1b4c19ba9240869bcc149fe4e2a032a59e176d3968f1d195ec07d6d163c28b5968c452d6f431fa74d4dfaa7c8f8b061d58ca3248d3cc7f819ffe92d034e
Type fulltextMimetype application/pdf

Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 1424 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 647 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf