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Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS).
2015 (engelsk)Inngår i: FEMS Microbiology Reviews, ISSN 0168-6445, E-ISSN 1574-6976, Vol. 39, nr 3, s. 428-441Artikkel, forskningsoversikt (Fagfellevurdert) Published
Abstract [en]

CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.

sted, utgiver, år, opplag, sider
2015. Vol. 39, nr 3, s. 428-441
Emneord [en]
crRNA biogenesis, Cas5d, Cas6, Cas9, tracrRNA, RNase III
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-106508DOI: 10.1093/femsre/fuv023ISI: 000355841200011OAI: oai:DiVA.org:umu-106508DiVA, id: diva2:841955
Tilgjengelig fra: 2015-07-15 Laget: 2015-07-14 Sist oppdatert: 2018-06-07bibliografisk kontrollert

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