umu.sePublikationer
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Visa övriga samt affilieringar
2019 (Engelska)Ingår i: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, nr 19, s. 5423-5436Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Maintaining mitochondrial proteome integrity is especially important under stress conditions to ensure a continued ATP supply for protection and adaptation responses in plants. Deg/HtrA proteases are important factors in the cellular protein quality control system, but little is known about their function in mitochondria. Here we analyzed the expression pattern and physiological function of Arabidopsis thaliana DEG10, which has homologs in all photosynthetic eukaryotes. Both expression of DEG10:GFP fusion proteins and immunoblotting after cell fractionation showed an unambiguous subcellular localization exclusively in mitochondria. DEG10 promoter:GUS fusion constructs showed that DEG10 is expressed in trichomes but also in the vascular tissue of roots and aboveground organs. DEG10 loss-of-function mutants were impaired in root elongation, especially at elevated temperature. Quantitative proteome analysis revealed concomitant changes in the abundance of mitochondrial respiratory chain components and assembly factors, which partially appeared to depend on altered mitochondrial retrograde signaling. Under field conditions, lack of DEG10 caused a decrease in seed production. Taken together, our findings demonstrate that DEG10 affects mitochondrial proteostasis, is required for optimal root development and seed set under challenging environmental conditions, and thus contributes to stress tolerance of plants.

Ort, förlag, år, upplaga, sidor
Oxford University Press, 2019. Vol. 70, nr 19, s. 5423-5436
Nyckelord [en]
Arabidopsis, Deg proteases, mitochondria, proteome, root, seed yield, temperature stress, G10, which has homologs in all photosynthetic eukaryotes. Both pression of DEG10:GFP fusion proteins and immunoblotting after cell actionation showed an unambiguous subcellular localization exclusively mitochondria. DEG10 promoter:GUS fusion constructs showed that DEG10 expressed in trichomes but also in the vascular tissue of roots and oveground organs. DEG10 loss-of-function mutants were impaired in root ongation, especially at elevated temperature. Quantitative proteome alysis revealed concomitant changes in the abundance of mitochondrial spiratory chain components and assembly factors, which partially peared to depend on altered mitochondrial retrograde signaling. Under eld conditions, lack of DEG10 caused a decrease in seed production. ken together, our findings demonstrate that DEG10 affects tochondrial proteostasis, is required for optimal root development and ed set under challenging environmental conditions, and thus ntributes to stress tolerance of plants.
Nationell ämneskategori
Botanik
Identifikatorer
URN: urn:nbn:se:umu:diva-164890DOI: 10.1093/jxb/erz294ISI: 000491240900029PubMedID: 31225599OAI: oai:DiVA.org:umu-164890DiVA, id: diva2:1368121
Forskningsfinansiär
Energimyndigheten, 2012-005889Tillgänglig från: 2019-11-06 Skapad: 2019-11-06 Senast uppdaterad: 2019-11-07Bibliografiskt granskad

Open Access i DiVA

fulltext(1979 kB)13 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1979 kBChecksumma SHA-512
ccb41e778cb4164c5ba8ddede6c7ac0ae06df74466afd8ec43f270ed4eab27e4d8580b81d61e5230e36580998b48f174bd72f25107f0cf0764c1ac6726a6f1a5
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Mishra, Laxmi S.Funk, Christiane

Sök vidare i DiVA

Av författaren/redaktören
Mishra, Laxmi S.Funk, Christiane
Av organisationen
Kemiska institutionen
I samma tidskrift
Journal of Experimental Botany
Botanik

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 13 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 30 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf