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Aromatic residues in the C terminus of apolipoprotein C-III mediate lipid binding and LPL inhibition
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
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2017 (Engelska)Ingår i: JOURNAL OF LIPID RESEARCH, Vol. 58, nr 5, s. 840-852Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Plasma apoC-III levels correlate with triglyceride (TG) levels and are a strong predictor of CVD outcomes. ApoC-III elevates TG in part by inhibiting LPL. ApoC-III likely inhibits LPL by competing for lipid binding. To probe this, we used oil-drop tensiometry to characterize binding of six apoC-III variants to lipid/water interfaces. This technique monitors the dependence of lipid binding on surface pressure, which increases during TG hydrolysis by LPL. ApoC-III adsorption increased surface pressure by upward of 18 mN/m at phospholipid/TG/water interfaces. ApoC-III was retained to high pressures at these interfaces, desorbing at 21-25 mN/m. Point mutants, which substituted alanine for aromatic residues, impaired the lipid binding of apoC-III. Adsorption and retention pressures decreased by 1-6 mN/m in point mutants, with the magnitude determined by the location of alanine substitutions. Trp42 was most critical to mediating lipid binding. These results strongly correlate with our previous results, linking apoC-III point mutants to increased LPL binding and activity at lipid surfaces.(jlr) We propose that aromatic residues in the C-terminal half of apoC-III mediate binding to TG-rich lipoproteins. Increased apoC-III expression in the hypertriglyceridemic state allows apoC-III to accumulate on lipoproteins and inhibit LPL by preventing binding and/or access to sub-strate.

Ort, förlag, år, upplaga, sidor
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC , 2017. Vol. 58, nr 5, s. 840-852
Nyckelord [en]
lipoprotein lipase, lipid and lipoprotein metabolism, LDL/metabolism, lipid/emulsions, protein-lipid teraction, surface pressure, drop tensiometry
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-136199DOI: 10.1194/jlr.M071126ISI: 000400479800004PubMedID: 28159869OAI: oai:DiVA.org:umu-136199DiVA, id: diva2:1120727
Tillgänglig från: 2017-07-07 Skapad: 2017-07-07 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Vorrsjo, EvelinaOlivecrona, Gunilla

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