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Retrospective genomic analysis of Vibrio cholerae O1 El Tor strains from different places in India reveals the presence of ctxB-7 allele found in Haitian isolates
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi. Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33 C. I. T. Road, Scheme XM, Beleghata, Kolkata-700010, India.
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2017 (Engelska)Ingår i: Epidemiology and Infection, ISSN 0950-2688, E-ISSN 1469-4409, Vol. 145, nr 11, s. 2212-2220Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A total of 45 strains of Vibrio cholerae O1 isolated from 10 different places in India where they were associated with cases of cholera between the years 2007 and 2008 were examined by molecular methods. With the help of phenotypic and genotypic tests the strains were confirmed to be O1 El Tor biotype strains with classical ctxB gene. Polymerase chain reaction (PCR) analysis by double - mismatch amplification mutation assay PCR showed 16 of these strains carried the ctxB-7 allele reported in Haitian strains. Sequencing of the ctxB gene in all the 45 strains revealed that in 16 strains the histidine at the 20th amino acid position had been replaced by asparagine and this single nucleotide polymorphism did not affect cholera toxin production as revealed by beads enzyme-linked immunosorbent assay. This study shows that the new ctxB gene sequence was circulating in different places in India. Seven representatives of these 45 strains analysed by pulsed - field gel electrophoresis showed four distinct Not I digested profiles showing that multiple clones were causing cholera in 2007 and 2008.

Ort, förlag, år, upplaga, sidor
Cambridge University Press, 2017. Vol. 145, nr 11, s. 2212-2220
Nyckelord [en]
ctxB-7, molecular typing, PFGE, Surveillance, Vibrio cholerae
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-139820DOI: 10.1017/S0950268817001182ISI: 000409124100005PubMedID: 28712383OAI: oai:DiVA.org:umu-139820DiVA, id: diva2:1144233
Tillgänglig från: 2017-09-25 Skapad: 2017-09-25 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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