umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandläkarutbildning. Department of Biochemistry, University of Turku, Turku, Finland.ORCID-id: 0000-0003-4118-0370
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandläkarutbildning. Department of Periodontics, Stomatological Hospital, Southern Medical University, Guangzhou, People’s Republic of China.
Visa övriga samt affilieringar
2018 (Engelska)Ingår i: Medical Microbiology and Immmunology, ISSN 0300-8584, E-ISSN 1432-1831, Vol. 207, nr 5-6, s. 329-338Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Phosphorylcholine (ChoP) is covalently incorporated into bacterial surface structures, contributing to host mimicry and promoting adhesion to surfaces. Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which surface structures bear ChoP, and whether ChoP-positivity relates to serum killing. The tested oral (N=67) and blood isolates (N=27) represented 6 serotypes. Mab TEPC-15 was used for immunoblotting of cell lysates and fractions and for immunofluorescence microscopy of cell surface-bound ChoP. The lysates were denatured with urea for hidden ChoP or treated with proteinase K to test whether it binds to a protein. Three ChoP-positive and two ChoP-negative strains were subjected to serum killing in the presence/absence of CRP and using Ig-depleted serum as complement source. Cell lysates and the first soluble cellular fraction revealed a<10kDa band in immunoblots. Among 94 strains, 27 were ChoP positive. No difference was found in the prevalence of ChoP-positive oral (21/67) and blood (6/27) strains. Immunofluorescence microscopy corresponded to the immunoblot results. Proteinase K abolished ChoP reactivity, whereas urea did not change the negative result. The TEPC-15-reactive protein was undetectable in flp1 mutant strain. The survival rate of serotype-b strains in serum was 100% irrespective of ChoP, but that of serotype-a was higher in ChoP-positive (85%) than ChoP-negative (71%) strains. The results suggest that a third of rough-colony strains harbor ChoP and that ChoP is attached to fimbrial subunit protein Flp1. It further seems that ChoP-positivity does not enhance but may reduce A. actinomycetemcomitans susceptibility to serum killing.

Ort, förlag, år, upplaga, sidor
Springer, 2018. Vol. 207, nr 5-6, s. 329-338
Nyckelord [en]
Aggregatibacter actinomycetemcomitans, Serotypes, Periodontitis, Phosphorylcholine, Fimbriae, Flp1, C-reactive protein, Serum resistance
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-153696DOI: 10.1007/s00430-018-0554-1ISI: 000447969100008PubMedID: 30056510OAI: oai:DiVA.org:umu-153696DiVA, id: diva2:1268262
Forskningsfinansiär
Västerbottens läns landstingTillgänglig från: 2018-12-05 Skapad: 2018-12-05 Senast uppdaterad: 2018-12-05Bibliografiskt granskad

Open Access i DiVA

fulltext(2449 kB)59 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 2449 kBChecksumma SHA-512
0605be8a2216d80ce07b0072900b234cec5e4f3e874c2ef2202b003233b60a44ce4c234c38bf4061adb58c5f1898cb42371211f3f3a0c2ac1a79394c22e4400c
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Ihalin, RiikkaAsikainen, Sirkka

Sök vidare i DiVA

Av författaren/redaktören
Ihalin, RiikkaAsikainen, Sirkka
Av organisationen
Tandläkarutbildning
I samma tidskrift
Medical Microbiology and Immmunology
Mikrobiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 59 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 252 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf