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Dissecting the microscopic steps of the cyclophilin a enzymatic cycle on the biological substrate HIV-capsid by NMR
Department of Biochemistry and Howard Hughes Medical Institute, Brandeis University, Waltham, MA, USA.
Department of Biochemistry and Howard Hughes Medical Institute, Brandeis University, Waltham, MA, USA.
Department of Biochemistry and Howard Hughes Medical Institute, Brandeis University, Waltham, MA, USA.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
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2010 (Engelska)Ingår i: Journal of Molecular Biology, ISSN 0022-2836, E-ISSN 1089-8638, Vol. 403, nr 5, s. 723-38Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Peptidyl-prolyl isomerases (PPIases) are emerging as key regulators of many diverse biological processes. Elucidating the role of PPIase activity in vivo has been challenging because mutagenesis of active site residues not only reduces the catalytic activity of these enzymes, but also dramatically affects substrate binding. Employing the cyclophilin A (CypA) PPIase together with its biologically relevant and natively folded substrate, the N-terminal domain of the HIV-1 capsid (CA(N)) protein, we demonstrate here how to dissect residue specific contributions to PPIase catalysis versus substrate binding utilizing NMR spectroscopy. Surprisingly, a number of CypA active-site mutants previously assumed to be strongly diminished in activity toward biological substrates based on a peptide assay only, catalyze the HIV capsid with wild-type activity, but with a change in the rate-limiting step of the enzymatic cycle. The results illustrate that a quantitative analysis of catalysis using the biological substrates is critical when interpreting the effects of PPIase mutations in biological assays.

Ort, förlag, år, upplaga, sidor
Elsevier , 2010. Vol. 403, nr 5, s. 723-38
Nyckelord [en]
cyclophilin A, prolyl isomerase, HIV-1 replication, NMR, backbone dynamics
Identifikatorer
URN: urn:nbn:se:umu:diva-35481DOI: 10.1016/j.jmb.2010.08.001PubMedID: 20708627OAI: oai:DiVA.org:umu-35481DiVA, id: diva2:344559
Tillgänglig från: 2010-08-19 Skapad: 2010-08-19 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Wolf-Watz, Magnus

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