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O-Linked glycosylation of the PilA pilin protein of francisella tularensis: identification of the endogenous protein-targeting oligosaccharyltransferase and characterization of the native oligosaccharide
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2011 (Engelska)Ingår i: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 193, nr 19, s. 5487-5497Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Findings from a number of studies suggest that the PilA pilin proteins may play an important role in the pathogenesis of disease caused by species within the genus Francisella. As such, a thorough understanding of PilA structure and chemistry is warranted. Here, we definitively identified the PglA protein-targeting oligosaccharyltransferase by virtue of its necessity for PilA glycosylation in Francisella tularensis and its sufficiency for PilA glycosylation in Escherichia coli. In addition, we used mass spectrometry to examine PilA affinity purified from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica and demonstrated that the protein undergoes multisite, O-linked glycosylation with a pentasaccharide of the structure HexNac-HexHex-HexNac-HexNac. Further analyses revealed microheterogeneity related to forms of the pentasaccharide carrying unusual moieties linked to the distal sugar via a phosphate bridge. Type A and type B strains of Francisella subspecies thus express an O-linked protein glycosylation system utilizing core biosynthetic and assembly pathways conserved in other members of the proteobacteria. As PglA appears to be highly conserved in Francisella species, O-linked protein glycosylation may be a feature common to members of this genus.

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Baltimore: Williams & Wilkins , 2011. Vol. 193, nr 19, s. 5487-5497
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Mikrobiologi
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URN: urn:nbn:se:umu:diva-48207DOI: 10.1128/JB.00383-11ISI: 000294826200047OAI: oai:DiVA.org:umu-48207DiVA, id: diva2:447207
Tillgänglig från: 2011-10-11 Skapad: 2011-10-10 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Forsberg, Åke

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Umeå Centre for Microbial Research (UCMR)
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Totalt: 195 träffar
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