umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Hsp70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID-id: 0000-0002-6181-9904
Visa övriga samt affilieringar
1998 (Engelska)Ingår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 95, nr 5, s. 2319-2324, artikel-id 9482883Artikel i tidskrift (Refereegranskat) Published
Fritextbeskrivning
Abstract [en]

In response to various stress stimuli, heat shock genes are induced to express heat shock proteins (Hsps). Previous studies have revealed that expression of heat shock genes is regulated both at transcriptional and posttranscriptional level, and the rapid transcriptional induction of heat shock genes involves activation of the specific transcription factor, heat shock factor 1 (HSF1). Furthermore, the transcriptional induction can vary in intensity and kinetics in a signal- and cell-type-dependent manner. In this study, we demonstrate that mechanical loading in the form of hydrostatic pressure increases heat shock gene expression in human chondrocyte-like cells. The response to continuous high hydrostatic pressure was characterized by elevated mRNA and protein levels of Hsp70, without activation of HSF1 and transcriptional induction of hsp70 gene. The increased expression of Hsp70 was mediated through stabilization of hsp70 mRNA molecules. Interestingly, in contrast to static pressurization, cyclic hydrostatic loading did not result in the induction of heat shock genes. Our findings show that hsp70 gene expression is regulated posttranscriptionally without transcriptional induction in chondrocyte-like cells upon exposure to high continuous hydrostatic pressure. We suggest that the posttranscriptional regulation in the form of hsp70 mRNA stabilization provides an additional mode of heat shock gene regulation that is likely to be of significant importance in certain forms of stress.

Ort, förlag, år, upplaga, sidor
1998. Vol. 95, nr 5, s. 2319-2324, artikel-id 9482883
Nyckelord [en]
Immortalized chondrocyte, hydrostatic pressure, stress response, heat shock protein 70, mRNA stabilization
Nationell ämneskategori
Cell- och molekylärbiologi Biokemi och molekylärbiologi
Forskningsämne
biokemi; cellforskning
Identifikatorer
URN: urn:nbn:se:umu:diva-107696PubMedID: 9482883OAI: oai:DiVA.org:umu-107696DiVA, id: diva2:849044
Tillgänglig från: 2015-08-27 Skapad: 2015-08-27 Senast uppdaterad: 2018-06-07

Open Access i DiVA

Fulltext saknas i DiVA

PubMed

Personposter BETA

Lammi, Mikko

Sök vidare i DiVA

Av författaren/redaktören
Lammi, Mikko
I samma tidskrift
Proceedings of the National Academy of Sciences of the United States of America
Cell- och molekylärbiologiBiokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetricpoäng

pubmed
urn-nbn
Totalt: 78 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf