Semiconstant-Time P,H-COSY NMR: Analysis of Complex Mixtures of Phospholipids Originating from Helicobacter pylori
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics
Umeå University, Faculty of Science and Technology, Department of Chemistry
Article in journal (Refereed)
ACS Publications ASAP
Journal of the American Chemical Society(ISSN 0002-7863)(EISSN 1520-5126)
Lipids play a central role in numerous biological events, ranging from normal physiological processes to host−pathogen interactions. The proposed semiconstant-time 31P,1H−COSY NMR experiment provides identification of known and structural characterization of unknown phospholipids in complex membrane extracts with high sensitivity, based on the combination of their 1H and 31P chemical shifts and coupling patterns. Furthermore, the spectra allow quantification of phospholipid composition. Analysis of the phospholipid composition of Helicobacter pylori, the causative agent of peptic ulcer disease, showed the presence of uncommon phospholipids. This novel NMR approach allows the study of changes in membrane composition in response to biological stimuli and opens up the possibility of identifying soluble phosphorus species in a number of research fields.
Publication Date (Web): September 17, 2009