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Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR).
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2016 (engelsk)Inngår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, nr 51, s. 14733-14738Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Infection by the human bacterial pathogen Listeria monocytogenes is mainly controlled by the positive regulatory factor A (PrfA), a member of the Crp/Fnr family of transcriptional activators. Published data suggest that PrfA requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (GSH) could fulfill this function. Here we report the crystal structures of PrfA in complex with GSH and in complex with GSH and its cognate DNA, the hly operator PrfA box motif. These structures reveal the structural basis for a GSH-mediated allosteric mode of activation of PrfA in the cytosol of the host cell. The crystal structure of PrfAWT in complex only with DNA confirms that PrfAWT can adopt a DNA binding-compatible structure without binding the GSH activator molecule. By binding to PrfA in the cytosol of the host cell, GSH induces the correct fold of the HTH motifs, thus priming the PrfA protein for DNA interaction.

sted, utgiver, år, opplag, sider
2016. Vol. 113, nr 51, s. 14733-14738
Emneord [en]
Listeria, PrfA, activation, glutathione, virulence
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-128915DOI: 10.1073/pnas.1614028114ISI: 000390044900062OAI: oai:DiVA.org:umu-128915DiVA, id: diva2:1057771
Tilgjengelig fra: 2016-12-19 Laget: 2016-12-19 Sist oppdatert: 2018-06-09bibliografisk kontrollert

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Hall, MichaelGrundström, ChristinBegum, AfshanLindberg, Mikael J.Sauer, Uwe H.Almqvist, FredrikJohansson, JörgenSauer-Eriksson, A. Elisabeth

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Hall, MichaelGrundström, ChristinBegum, AfshanLindberg, Mikael J.Sauer, Uwe H.Almqvist, FredrikJohansson, JörgenSauer-Eriksson, A. Elisabeth
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