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Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
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2017 (Engelska)Ingår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 987, s. 15-24Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The EIS measurements of MTX resulted in a data matrix of size 39 × 55. The data were analysed using multivariate data analysis and showed a concentration dependence and time dependence that could be separated. This allowed the calculation of a multivariate calibration model. The model showed good linear behavior on a logarithmic scale offering a detection limit of 5 × 10−12 mol L−1.

Ort, förlag, år, upplaga, sidor
Elsevier, 2017. Vol. 987, s. 15-24
Nyckelord [en]
Gold electrode, Biosensor, X-ray photoelectron spectroscopy, Singular value decomposition, Multivariate calibration model, Antibody
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:umu:diva-138962DOI: 10.1016/j.aca.2017.08.034ISI: 000410304700002PubMedID: 28916036OAI: oai:DiVA.org:umu-138962DiVA, id: diva2:1138350
Tillgänglig från: 2017-09-05 Skapad: 2017-09-05 Senast uppdaterad: 2019-11-07Bibliografiskt granskad
Ingår i avhandling
1. Development of electrochemical sensor and biosensor platforms: detection of therapeutic drugs and heavy metal ions
Öppna denna publikation i ny flik eller fönster >>Development of electrochemical sensor and biosensor platforms: detection of therapeutic drugs and heavy metal ions
2019 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Electrochemical sensors and biosensors combine the sensitivity of electroanalytical methods with the selectivity of a sensor or biosensor surface. The chemical or biochemical component (receptor) in the sensor recognizes an analyte and produces an electrical signal which is proportional to the analyte concentration. Some of these sensors are routinely used in clinical applications and are known for their simplicity, portability, cost-effective, and miniaturization. The glucose sensor used in the management of diabetes is a good example of such biosensors.

This thesis deals with the development of electrochemical biosensor and sensor platforms for the detection of therapeutic drugs, demonstrated using methotrexate (MTX) which is the most common drug used for the treatment of cancer patients, and heavy metal ions (Pb2+ and Cd2+).

The biosensor surfaces were generated by immobilization of antibody (anti-MTX) on chemically modified gold electrodes using different surface modification protocols. Self-assemble monolayer (SAM) using alkanethiol (cysteamine) or electrografting with diazonium salt (4- carboxybenzenediaonium tetrafluoroborate, 4-CBD) was used for surface modification. The surface modification was monitored and characterized using electrochemical immittance spectroscopy (EIS) and cyclic voltammetry (CV) along with other complementary technique such as X-ray photoelectron spectroscopy (XPS). The biosensing surfaces were used for the detection of MTX in an electrochemical flow cell (paper I) and in a batch system (paper II). The detection was based on non-faradaic electrochemical immittance spectroscopy (EIS) and singular value decomposition (SVD) for data evaluation. Both electrochemical biosensors provided the lowest limit of detection, LOD (at picomolar level) compared to earlier reports.

The electrografting of 4-CBD on glassy carbon electrode (GCE) using CV and the parameters that influence the number of monolayers that can be grafted on the surface are demonstrated (paper III). The CVs obtained during grafting showed one or two reduction peaks, and this was found to be related to the number of monolayers deposited on the electrode. One can increase the number of monolayers by increasing the concentration of 4-CBD or decreasing the scan rate. The GCE, grafted using 4-CBD, was incorporated with Bi by an in situ electrodeposition of Bi3+ and used as an electrochemical sensor for detection of Pb2+ and Cd2+ using square wave anodic stripping voltammetry, SWASV (paper IV). The sensor resulted in LOD of 10 μg L-1 for Pb2+ and 25 μg L-1 for Cd2+. The applicability of the sensor was tested for detection of Pb2+ and Cd2+ in tap water and compared with ICP-OES. The results were comparable, demonstrating the potential of the sensor as an alternative to ICP-OES for the detection of metal ions in water samples.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2019. s. 64
Nyckelord
Sensors, electrode surface modification, electrochemical immitance spectroscopy, square wave anodic stripping voltammetry, singular value decomposition, therapeutic drugs, heavy metals
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:umu:diva-165049 (URN)978-91-7855-149-1 (ISBN)
Disputation
2019-12-13, KB.E3.01 (Lilla hörsalen), KBC-huset, Umeå, 10:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2019-11-11 Skapad: 2019-11-07 Senast uppdaterad: 2019-11-08Bibliografiskt granskad

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Phal, SereilakhenaLindholm-Sethson, BrittaShchukarev, AndreyTesfalidet, Solomon

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Analytica Chimica Acta
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