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Protein expression in tension wood formation monitored at high tissue resolution in Populus
Umeå University, Faculty of Science and Technology, Department of Chemistry. (Computational Life Science Cluster (CLiC))
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2017 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 68, no 13, p. 3405-3417Article in journal (Refereed) Published
Abstract [en]

Tension wood (TW) is a specialized tissue with contractile properties that is formed by the vascular cambium in response to gravitational stimuli. We quantitatively analysed the proteomes of Populus tremula cambium and its xylem cell derivatives in stems forming normal wood (NW) and TW to reveal the mechanisms underlying TW formation. Phloem-, cambium-, and wood-forming tissues were sampled by tangential cryosectioning and pooled into nine independent samples. The proteomes of TW and NW samples were similar in the phloem and cambium samples, but diverged early during xylogenesis, demonstrating that reprogramming is an integral part of TW formation. For example, 14-3-3, reactive oxygen species, ribosomal and ATPase complex proteins were found to be up-regulated at early stages of xylem differentiation during TW formation. At later stages of xylem differentiation, proteins involved in the biosynthesis of cellulose and enzymes involved in the biosynthesis of rhamnogalacturonan-I, rhamnogalacturonan-II, arabinogalactan-II and fasciclin-like arabinogalactan proteins were up-regulated in TW. Surprisingly, two isoforms of exostosin family proteins with putative xylan xylosyl transferase function and several lignin biosynthesis proteins were also up-regulated, even though xylan and lignin are known to be less abundant in TW than in NW. These data provided new insight into the processes behind TW formation.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 68, no 13, p. 3405-3417
Keywords [en]
cell wall, cellulose, lignin, Populus, proteomics, tension wood, tissue resolution, xylogenesis
National Category
Plant Biotechnology Botany
Identifiers
URN: urn:nbn:se:umu:diva-139165DOI: 10.1093/jxb/erx186ISI: 000408119100011OAI: oai:DiVA.org:umu-139165DiVA, id: diva2:1139704
Available from: 2017-09-08 Created: 2017-09-08 Last updated: 2018-06-09Bibliographically approved

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Bygdell, JoakimTrygg, Johan

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