umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Environment-dependent regulation of spliceosome activity by the LSM2-8 complex in Arabidopsis
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Departamento de Biolog´ıa Medioambiental, Centro de Investigaciones Biologicas, CSIC, 28040 Madrid, Spain.
Show others and affiliations
2017 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 45, no 12, p. 7416-7431Article in journal (Refereed) Published
Abstract [en]

Spliceosome activity is tightly regulated to ensure adequate splicing in response to internal and external cues. It has been suggested that core components of the spliceosome, such as the snRNPs, would participate in the control of its activity. The experimental indications supporting this proposition, however, remain scarce, and the operating mechanisms poorly understood. Here, we present genetic and molecular evidence demonstrating that the LSM2-8 complex, the protein moiety of the U6 snRNP, regulates the spliceosome activity in Arabidopsis, and that this regulation is controlled by the environmental conditions. Our results show that the complex ensures the efficiency and accuracy of constitutive and alternative splicing of selected pre-mRNAs, depending on the conditions. Moreover, miss-splicing of most targeted pre-mRNAs leads to the generation of nonsense mediated decay signatures, indicating that the LSM2-8 complex also guarantees adequate levels of the corresponding functional transcripts. Interestingly, the selective role of the complex has relevant physiological implications since it is required for adequate plant adaptation to abiotic stresses. These findings unveil an unanticipated function for the LSM2-8 complex that represents a new layer of posttranscriptional regulation in response to external stimuli in eukaryotes.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 45, no 12, p. 7416-7431
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-138541DOI: 10.1093/nar/gkx375ISI: 000404879000041OAI: oai:DiVA.org:umu-138541DiVA, id: diva2:1141707
Available from: 2017-09-15 Created: 2017-09-15 Last updated: 2018-06-09Bibliographically approved

Open Access in DiVA

fulltext(4015 kB)77 downloads
File information
File name FULLTEXT01.pdfFile size 4015 kBChecksum SHA-512
dfd5542099617974a8574554e5a2bad140fcdb29485faa667f24cf1d37a300c584a70bc731d97886eda7e03a7b5d3958fa1d958e0d4de2048ba3b8124ed46284
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Hernandez-Verdeja, Tamara

Search in DiVA

By author/editor
Hernandez-Verdeja, Tamara
By organisation
Umeå Plant Science Centre (UPSC)Department of Plant Physiology
In the same journal
Nucleic Acids Research
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 77 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 172 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf