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Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Max Planck Institute for Developmental Biology, Department of Molecular Biology, Spemannstr. 35, 72076 Tübingen, Germany.
2017 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 68, no 18, p. 5117-5127Article in journal (Refereed) Published
Abstract [en]

FLOWERING LOCUS M (FLM), a component of the thermosensory flowering time pathway in Arabidopsis thaliana, is regulated by temperature-dependent alternative splicing (AS). The main splicing variant, FLM-beta, is a well-documented floral repressor that is down-regulated in response to increasing ambient growth temperature. Two hypotheses have been formulated to explain how flowering time is modulated by AS of FLM. In the first model a second splice variant, FLM-delta, acts as a dominant negative isoform that competes with FLM-beta at elevated ambient temperatures, thereby indirectly promoting flowering. Alternatively, it has been suggested that the induction of flowering at elevated temperatures is caused only by reduced FLM-beta expression. To better understand the role of the two FLM splice forms, we employed CRISPR/Cas9 technology to specifically delete the exons that characterize each splice variant. Lines that produced repressive FLM-beta but were incapable of producing FLM-delta were late flowering. In contrast, FLM-beta knockout lines that still produced FLM-delta flowered early, but not earlier than the flm-3 loss of function mutant, as would be expected if FLM-delta had a dominant-negative effect on flowering. Our data support the role of FLM-beta as a flower repressor and provide evidence that a contribution of FLM-delta to the regulation of flowering time in wild-type A. thaliana seems unlikely.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 68, no 18, p. 5117-5127
Keywords [en]
Arabidopsis thaliana, CRISPR/Cas9, FLOWERING LOCUS M (FLM), flowering time, splice isoforms, temperature-dependent alternative splicing
National Category
Genetics and Breeding in Agricultural Sciences
Identifiers
URN: urn:nbn:se:umu:diva-142004DOI: 10.1093/jxb/erx328ISI: 000414371700011PubMedID: 29036339OAI: oai:DiVA.org:umu-142004DiVA, id: diva2:1158935
Available from: 2017-11-21 Created: 2017-11-21 Last updated: 2018-06-09Bibliographically approved

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Schmid, Markus

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