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Finke-Watzky Two-Step Nucleation-Autocatalysis Model of S100A9 Amyloid Formation: Protein Misfolding as "Nucleation" Event
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik. Department of General Chemistry, Sumy State University, Sumy, Ukraine.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik. Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.
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2017 (Engelska)Ingår i: ACS Chemical Neuroscience, ISSN 1948-7193, E-ISSN 1948-7193, Vol. 8, nr 10, s. 2152-2158Artikel i tidskrift, Letter (Refereegranskat) Published
Abstract [en]

Quantitative kinetic analysis is critical for understanding amyloid mechanisms. Here we demonstrate the application of generic Finke-Watzky (F-W) two-step nucleation-autocatalytic growth model to the concentration-dependent amyloid kinetics of proinflammatory alpha-helical S100A9 protein at pH 7.4 and at 37 and 42 degrees C. The model is based on two pseudoelementary reaction steps applied without further analytical constraints, and its treatment of S100A9 amyloid self-assembly demonstrates that initial misfolding and beta-sheet formation, defined as "nucleation" step, spontaneously takes place within individual S100A9 molecules at higher rate than the subsequent fibrillar growth. The latter, described as an autocatalytic process, will proceed if misfolded amyloid-prone S100A9 is populated on a macroscopic time scale. Short lengths of S100A9 fibrils are consistent with the F-W model. The analysis of fibrillar length distribution by the Beker-Doring model demonstrates independently that such distribution is solely determined by slow fibril growth and there is no fragmentation or secondary pathways decreasing fibrillar length.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2017. Vol. 8, nr 10, s. 2152-2158
Nyckelord [en]
Amyloid, autocatalysis, Finke-Watzky model, growth, kinetics, nucleation, S100A9
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) Neurovetenskaper
Identifikatorer
URN: urn:nbn:se:umu:diva-141827DOI: 10.1021/acschemneuro.7b00251ISI: 000413502700010PubMedID: 28759719OAI: oai:DiVA.org:umu-141827DiVA, id: diva2:1160445
Tillgänglig från: 2017-11-27 Skapad: 2017-11-27 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Iashchishyn, Igor A.Sulskis, DariusMorozova-Roche, Ludmilla A.

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Iashchishyn, Igor A.Sulskis, DariusMorozova-Roche, Ludmilla A.
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ACS Chemical Neuroscience
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)Neurovetenskaper

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