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Random Initialisation of the Spectral Variables: an Alternate Approach for Initiating Multivariate Curve Resolution Alternating Least Square (MCR-ALS) Analysis
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2017 (English)In: Journal of Fluorescence, ISSN 1053-0509, E-ISSN 1573-4994, Vol. 27, no 6, p. 1957-1968Article in journal (Refereed) Published
Abstract [en]

Multivariate curve resolution alternating least square (MCR-ALS) analysis is the most commonly used curve resolution technique. The MCR-ALS model is fitted using the alternate least square (ALS) algorithm that needs initialisation of either contribution profiles or spectral profiles of each of the factor. The contribution profiles can be initialised using the evolve factor analysis; however, in principle, this approach requires that data must belong to the sequential process. The initialisation of the spectral profiles are usually carried out using the pure variable approach such as SIMPLISMA algorithm, this approach demands that each factor must have the pure variables in the data sets. Despite these limitations, the existing approaches have been quite a successful for initiating the MCR-ALS analysis. However, the present work proposes an alternate approach for the initialisation of the spectral variables by generating the random variables in the limits spanned by the maxima and minima of each spectral variable of the data set. The proposed approach does not require that there must be pure variables for each component of the multicomponent system or the concentration direction must follow the sequential process. The proposed approach is successfully validated using the excitation-emission matrix fluorescence data sets acquired for certain fluorophores with significant spectral overlap. The calculated contribution and spectral profiles of these fluorophores are found to correlate well with the experimental results. In summary, the present work proposes an alternate way to initiate the MCR-ALS analysis.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 27, no 6, p. 1957-1968
Keywords [en]
MCR-ALS, EEMF random initialisation, Alternate least square, Contribution matrix, Spectral matrix, Factors
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-141795DOI: 10.1007/s10895-017-2132-0ISI: 000413690400004PubMedID: 28646301OAI: oai:DiVA.org:umu-141795DiVA, id: diva2:1161325
Available from: 2017-11-29 Created: 2017-11-29 Last updated: 2018-06-09Bibliographically approved

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Kumar, Keshav

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