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Amperometric biosensor for formic acid in air
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational Medicine. Cranfield Biotechnology Centre, Cranfield University, UK; Department of Chemistry, National Institute for Working Life, Umeå, Sweden.
Cranfield Biotechnology Centre, Cranfield University, UK.
Department of Chemistry, National Institute for Working Life, Umeå, Sweden.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational Medicine. Department of Chemistry, National Institute for Working Life, Umeå, Sweden.
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2000 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 70, no 1–3, p. 182-187Article in journal (Refereed) Published
Abstract [en]

The possibility of developing a simple, inexpensive and specific personal passive “real-time” air sampler incorporating a biosensor for formic acid was investigated. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase (FDH) with nicotinamide adenine dinucleotide (NAD+) as a co-factor and Meldola's blue as mediator. An effective way to immobilise the enzyme, co-factor and Meldola's blue on screen-printed, disposable, electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. Steady-state current was reached after 4–15 min and the limit of detection was calculated to be below 1 mg/m3. However, the response decreased by 50% after storage at −15°C for 1 day.

Place, publisher, year, edition, pages
Elsevier, 2000. Vol. 70, no 1–3, p. 182-187
Keywords [en]
Biosensor, Air monitoring, Formic acid, Gas-permeable membrane, Glycerol
National Category
Other Environmental Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-149303DOI: 10.1016/S0925-4005(00)00566-9ISI: 000090126600026OAI: oai:DiVA.org:umu-149303DiVA, id: diva2:1220769
Available from: 2011-02-04 Created: 2018-06-19Bibliographically approved

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