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Characterization of clinically relevant model bacterial strains of Pseudomonas aeruginosa for anti-biofilm testing of materials
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2018 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 76, p. 99-107Article in journal (Refereed) Published
Abstract [en]

There is a great interest in developing novel anti-biofilm materials in order to decrease medical device-associated bacterial infections causing morbidity and high healthcare costs. However, the testing of novel materials is often done using bacterial lab strains that may not exhibit the same phenotype as clinically relevant strains infecting medical devices. Furthermore, no consensus of strain selection exists in the field, making results very difficult to compare between studies. In this work, 19 clinical isolates of Pseudomonas aeruginosa originating from intubated patients in an intensive care unit have been characterized and compared to the lab reference strain PAO1 and a rmlC lipopolysaccharide mutant of PAO1. The adhesion and biofilm formation was monitored, as well as cell properties such as hydrophobicity, zeta potential and motility. Two groups of isolates were observed: one with high adhesion to polymer surfaces and one with low adhesion (the latter including PAO1). Furthermore, detailed biofilm assays in a flow system were performed using five characteristic isolates from the two groups. Confocal microscopy showed that the adhesion and biofilm formation of four of these five strains could be reduced dramatically on zwitterionic surface coatings. However, one isolate with pronounced swarming colonized and formed biofilm also on the antifouling surface. We demonstrate that the biofilm properties of clinical isolates can differ greatly from that of a standard lab strain and propose two clinical model strains for testing of materials designed for prevention of biofilm formation in the respiratory tract. The methodology used could beneficially be applied for screening of other collections of pathogens to identify suitable model strains for in vitro biofilm testing.

Statement of Significance: Medical-device associated infections present a great challenge in health care. Therefore, much research is undertaken to prevent bacterial colonization of new types of biomaterials. The work described here characterizes, tests and presents a number of clinically relevant bacterial model strains for assessing biofilm formation by Pseudomonas aeruginosa. Such model strains are of importance as they may provide better predictability of lab testing protocols with respect to how well materials would perform in an infection situation in a patient. Furthermore, this study uses the strains to test the performance of polymer surfaces designed to repel bacterial adhesion and it is shown that the biofilm formation for four out of the five tested bacterial strains was reduced.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 76, p. 99-107
Keywords [en]
Clinical isolates, Pseudomonas aeruginosa, Antifouling, Model strain, Surface chemistry
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Identifiers
URN: urn:nbn:se:umu:diva-150151DOI: 10.1016/j.actbio.2018.06.019ISI: 000442055600010PubMedID: 29902594Scopus ID: 2-s2.0-8504885706OAI: oai:DiVA.org:umu-150151DiVA, id: diva2:1232493
Funder
Stiftelsen Olle Engkvist Byggmästare, 2014/660Available from: 2018-07-11 Created: 2018-07-11 Last updated: 2018-09-10Bibliographically approved

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The full text will be freely available from 2020-06-12 00:00
Available from 2020-06-12 00:00

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Rzhepishevska, Olena I.Lundquist, MargarethaHakobyan, ShoghikSjöstedt, AndersRamstedt, Madeleine

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Rzhepishevska, Olena I.Lundquist, MargarethaHakobyan, ShoghikSjöstedt, AndersRamstedt, Madeleine
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