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Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome
Department of Forest Genetics and Plant Physiology, SLU, Umeå Plant Science Centre (UPSC), Umeå, Sweden.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Department of Forest Genetics and Plant Physiology, SLU, Umeå Plant Science Centre (UPSC), Umeå, Sweden.
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2018 (English)In: New Phytologist, ISSN 0028-646X, Vol. 219, no 1, p. 230-245Article in journal (Refereed) Published
Abstract [en]

Xylan is one of the main compounds determining wood properties in hardwood species. The xylan backbone is thought to be synthesized by a synthase complex comprising two members of the GT43 family. We downregulated all GT43 genes in hybrid aspen (Populus tremulaxtremuloides) to understand their involvement in xylan biosynthesis.

All three clades of the GT43 family were targeted for downregulation using RNA interference individually or in different combinations, either constitutively or specifically in developing wood.

Simultaneous downregulation in developing wood of the B (IRX9) and C (IRX14) clades resulted in reduced xylan Xyl content relative to reducing end sequence, supporting their role in xylan backbone biosynthesis. This was accompanied by a higher lignocellulose saccharification efficiency. Unexpectedly, GT43 suppression in developing wood led to an overall growth stimulation, xylem cell wall thinning and a shift in cellulose orientation. Transcriptome profiling of these transgenic lines indicated that cell cycling was stimulated and secondary wall biosynthesis was repressed. We suggest that the reduced xylan elongation is sensed by the cell wall integrity surveying mechanism in developing wood.

Our results show that wood-specific suppression of xylan-biosynthetic GT43 genes activates signaling responses, leading to increased growth and improved lignocellulose saccharification.

Place, publisher, year, edition, pages
2018. Vol. 219, no 1, p. 230-245
Keywords [en]
cellulose microfibril angle, GT43, Populus, saccharification, secondary wall, wood development, xylan biosynthesis
National Category
Plant Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-150384DOI: 10.1111/nph.15160ISI: 000434153200026PubMedID: 29708593Scopus ID: 2-s2.0-85046148362OAI: oai:DiVA.org:umu-150384DiVA, id: diva2:1236938
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Bio4EnergyAvailable from: 2018-08-06 Created: 2018-08-06 Last updated: 2019-08-30Bibliographically approved

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Terebieniec, Barbara K.Winestrand, SandraDerba-Maceluch, MartaSchiffthaler, BastianStreet, NathanielJönsson, Leif J.

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Terebieniec, Barbara K.Winestrand, SandraDerba-Maceluch, MartaSchiffthaler, BastianStreet, NathanielJönsson, Leif J.
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Department of ChemistryDepartment of Plant Physiology
Plant Biotechnology

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