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Experimental Infection and Transmission Competence of Sindbis Virus in Culex torrentium and Culex pipiens Mosquitoes from Northern Sweden
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Section of Virology. Umeå University, Arctic Research Centre at Umeå University.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
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2019 (English)In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 19, no 2, p. 128-133Article in journal (Refereed) Published
Abstract [en]

Introduction: Sindbis virus (SINV) is a mosquito-borne Alphavirus known to infect birds and cause intermittent outbreaks among humans in Fenno-Scandia. In Sweden, the endemic area has mainly been in central Sweden. Recently, SINV infections have emerged to northern Sweden, but the vectorial efficiency for SINV of mosquito species in this northern region has not yet been ascertained.

Objective: Mosquito larvae were sampled from the Umea region in northern Sweden and propagated in a laboratory to adult stage to investigate the infection, dissemination, and transmission efficiency of SINV in mosquitoes.

Materials and Methods: The mosquito species were identified by DNA barcoding of the cytochrome oxidase I gene. Culex torrentium was the most abundant (82.2%) followed by Culex pipiens (14.4%), Aedes annulipes (1.1%), Anopheles claviger (1.1%), Culiseta bergrothi (1.1%), or other unidentified species (1.1%). Mosquitoes were fed with SINV-infected blood and monitored for 29 days to determine the viral extrinsic incubation period. Infection and dissemination were determined by RT-qPCR screening of dissected body parts of individual mosquitoes. Viral transmission was determined from saliva collected from individual mosquitoes at 7, 14, and 29 days. SINV was detected by cell culture using BHK-21 cells, RT-qPCR, and sequencing.

Results: Cx. torrentium was the only mosquito species in our study that was able to transmit SINV. The overall transmission efficiency of SINV in Cx. torrentium was 6.8%. The rates of SINV infection, dissemination, and transmission in Cx. torrentium were 11%, 75%, and 83%, respectively.

Conclusions: Cx. torrentium may be the key vector involved in SINV transmission in northern Sweden.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2019. Vol. 19, no 2, p. 128-133
Keywords [en]
Sindbis virus, Alphavirus, dissemination, transmission, Culex mosquitoes
National Category
Zoology
Identifiers
URN: urn:nbn:se:umu:diva-152980DOI: 10.1089/vbz.2018.2311ISI: 000446699100001PubMedID: 30300110Scopus ID: 2-s2.0-85060807212OAI: oai:DiVA.org:umu-152980DiVA, id: diva2:1260234
Funder
Swedish Research Council Formas, 221-2014-1556Available from: 2018-11-01 Created: 2018-11-01 Last updated: 2019-05-20Bibliographically approved

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Lwande, Olivia WesulaAhlm, ClasEvander, Magnus

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