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ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing
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2018 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 115, no 45, p. E10778-E10787Article in journal (Refereed) Published
Abstract [en]

Matching ATP: NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP: NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in living Arabidopsis thaliana seedlings, we found that MgATP(2-) concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) in Arabidopsis mesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP: NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.

Place, publisher, year, edition, pages
National Academy of Sciences , 2018. Vol. 115, no 45, p. E10778-E10787
Keywords [en]
ATP, chloroplasts, cytosol, mitochondria, photosynthesis
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-153646DOI: 10.1073/pnas.1711497115ISI: 000449459000031PubMedID: 30352850OAI: oai:DiVA.org:umu-153646DiVA, id: diva2:1266062
Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2018-11-27Bibliographically approved

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Gardeström, Per

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