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Plasma membrane damage caused by listeriolysin O is not repaired through endocytosis of the membrane pore
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
2018 (English)In: Biology Open, ISSN 2046-6390, Vol. 7, no 10Article in journal (Refereed) Published
Abstract [en]

Endocytic mechanisms have been suggested to be important for plasma membrane repair in response to pore-forming toxins such as listeriolysin O (LLO), which form membrane pores that disrupt cellular homeostasis. Yet, little is known about the specific role of distinct endocytic machineries in this process. Here, we have addressed the importance of key endocytic pathways and developed reporter systems for real-time imaging of the endocytic response to LLO pore formation. We found that loss of clathrin-independent endocytic pathways negatively influenced the efficiency of membrane repair. However, we did not detect any increased activity of these pathways, or co-localisation with the toxin or markers of membrane repair, suggesting that they were not directly involved in removal of LLO pores from the plasma membrane. In fact, markers of clathrin-independent carriers (CLICs) were rapidly disassembled in the acute phase of membrane damage due to Ca2+ influx, followed by a reassembly about 2 min after pore formation. We propose that these endocytic mechanisms might influence membrane repair by regulating the plasma membrane composition and tension, but not via direct internalisation of LLO pores.

Place, publisher, year, edition, pages
The Company of Biologists , 2018. Vol. 7, no 10
Keywords [en]
Membrane pore, Repair, Membrane damage, LLO, Listeriolysin, Caveolae, Clathrin-mediated endocytosis, Clathrin-independent endocytosis, CLIC
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-153708DOI: 10.1242/bio.035287ISI: 000448607800009PubMedID: 30254077OAI: oai:DiVA.org:umu-153708DiVA, id: diva2:1268181
Funder
Swedish Research CouncilSwedish Foundation for Strategic Research The Kempe FoundationsAvailable from: 2018-12-05 Created: 2018-12-05 Last updated: 2018-12-05Bibliographically approved

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Nygård Skalman, LarsHolst, Mikkel R.Larsson, ElinLundmark, Richard

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Nygård Skalman, LarsHolst, Mikkel R.Larsson, ElinLundmark, Richard
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Department of Medical Biochemistry and BiophysicsDepartment of Integrative Medical Biology (IMB)Molecular Infection Medicine Sweden (MIMS)
Microbiology

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