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Development and validation of an in vitro model system to study peripheral sensory neuron development and injury
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery. Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).ORCID iD: 0000-0003-2596-5936
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 15961Article in journal (Refereed) Published
Abstract [en]

The ability to discriminate between diverse types of sensation is mediated by heterogeneous populations of peripheral sensory neurons. Human peripheral sensory neurons are inaccessible for research and efforts to study their development and disease have been hampered by the availability of relevant model systems. The in vitro differentiation of peripheral sensory neurons from human embryonic stem cells therefore provides an attractive alternative since an unlimited source of biological material can be generated for studies that specifically address development and injury. The work presented in this study describes the derivation of peripheral sensory neurons from human embryonic stem cells using small molecule inhibitors. The differentiated neurons express canonical- and modality-specific peripheral sensory neuron markers with subsets exhibiting functional properties of human nociceptive neurons that include tetrodotoxin-resistant sodium currents and repetitive action potentials. Moreover, the derived cells associate with human donor Schwann cells and can be used as a model system to investigate the molecular mechanisms underlying neuronal death following peripheral nerve injury. The quick and efficient derivation of genetically diverse peripheral sensory neurons from human embryonic stem cells offers unlimited access to these specialised cell types and provides an invaluable in vitro model system for future studies.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018. Vol. 8, article id 15961
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Neurosciences
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URN: urn:nbn:se:umu:diva-153701DOI: 10.1038/s41598-018-34280-3ISI: 000448589200037PubMedID: 30374154OAI: oai:DiVA.org:umu-153701DiVA, id: diva2:1268238
Funder
Swedish Research Council, 22292Gunvor och Josef Anérs stiftelseVästerbotten County CouncilAvailable from: 2018-12-05 Created: 2018-12-05 Last updated: 2018-12-05Bibliographically approved

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Jones, IwanYelhekar, Tushar D.Wiberg, RebeccaKingham, Paul J.Johansson, StaffanWiberg, MikaelCarlsson, Leif

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Jones, IwanYelhekar, Tushar D.Wiberg, RebeccaKingham, Paul J.Johansson, StaffanWiberg, MikaelCarlsson, Leif
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Umeå Centre for Molecular Medicine (UCMM)Department of Integrative Medical Biology (IMB)Hand Surgery
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