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FLO1, FLO5 and FLO11 flocculation gene Expression impacts saccharomyces cerevisiae attachment to penicillium chrysogenum in a Co-immobilization technique
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Microbiology, University of Córdoba, Córdoba, Spain; Department of Chemistry, Umeå University, Umeå, Sweden.
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2018 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 9, article id 2586Article in journal (Refereed) Published
Abstract [en]

A reoccurring flaw of most yeast immobilization systems that limits the potential of the technique is leakage of the cells from the matrix. Leakage may be due to weakly adherent cells, deterioration of the matrix, or to new growth and loss of non-adherent daughter cells. Yeast biocapsules are a spontaneous, cost effective system of immobilization whereby Saccharomyces cerevisiae cells are attached to the hyphae of Penicillium chrysogenum, creating hollow spheres that allow recovery and reutilization. This attachment is based on naturally occurring adherent properties of the yeast cell surface. We hypothesized that proteins associated with flocculation might play a role in adherence to fungal hyphae. To test this hypothesis, yeast strains with overexpressed and deleted flocculation genes (FLO1, FLO5, and FLO11) were evaluated for biocapsule formation to observe the impact of gene expression on biocapsule diameter, number, volume, dry mass, and percent immobilized versus non-immobilized cells. Overexpression of all three genes enhanced immobilization and resulted in larger diameter biocapsules. In particular, overexpression of FLO11 resulted in a five fold increase of absorbed cells versus the wild type isogenic strain. In addition, deletion of FLO1 and FLO11 significantly decreased the number of immobilized yeast cells compared to the wild type BY4742. These results confirm the role of natural adherent properties of yeast cells in attachment to fungal hyphae and offer the potential to create strongly adherent cells that will produce adherent progeny thereby reducing the potential for cell leakage from the matrix.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2018. Vol. 9, article id 2586
Keywords [en]
FLO gene, Penicillium chrysogenum, co-immobilization, flocculation, Saccharomyces cerevisiae
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-153699DOI: 10.3389/fmicb.2018.02586ISI: 000448881300001PubMedID: 30429833OAI: oai:DiVA.org:umu-153699DiVA, id: diva2:1268257
Available from: 2018-12-05 Created: 2018-12-05 Last updated: 2018-12-05Bibliographically approved

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Moreno-Garcia, Jaime

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