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RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B
Vise andre og tillknytning
2018 (engelsk)Inngår i: RNA Biology, ISSN 1547-6286, E-ISSN 1555-8584, Vol. 15, nr 10, s. 1336-1347Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′ untranslated region of speB mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity in vivo, we mutated it and demonstrate that the presence of this G residue is essential for the processing of the speB mRNA 5′ UTR by RNase Y. Although RNase Y directly targets and processes speB, we show that RNase Y-mediated regulation of speB expression occurs primarily at the transcriptional level and independently of the processing in the speB mRNA 5′ UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the speB 5′ UTR and on the role of RNase Y in speB regulation.

sted, utgiver, år, opplag, sider
Taylor & Francis, 2018. Vol. 15, nr 10, s. 1336-1347
Emneord [en]
Streptococcus pyogenes, speB, RNase Y, virulence, 5' untranslated region, transcriptional regulation
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-154094DOI: 10.1080/15476286.2018.1532253ISI: 000450608900008PubMedID: 30290721Scopus ID: 2-s2.0-85055547433OAI: oai:DiVA.org:umu-154094DiVA, id: diva2:1269882
Forskningsfinansiär
Göran Gustafsson Foundation for Research in Natural Sciences and MedicineThe Kempe FoundationsSwedish Research Council, K2013-57X-21436-04-3Tilgjengelig fra: 2018-12-11 Laget: 2018-12-11 Sist oppdatert: 2023-03-23bibliografisk kontrollert

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Le Rhun, AnaïsCharpentier, Emmanuelle

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