umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B
Vise andre og tillknytning
2018 (engelsk)Inngår i: RNA Biology, ISSN 1547-6286, E-ISSN 1555-8584, Vol. 15, nr 10, s. 1336-1347Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′ untranslated region of speB mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity in vivo, we mutated it and demonstrate that the presence of this G residue is essential for the processing of the speB mRNA 5′ UTR by RNase Y. Although RNase Y directly targets and processes speB, we show that RNase Y-mediated regulation of speB expression occurs primarily at the transcriptional level and independently of the processing in the speB mRNA 5′ UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the speB 5′ UTR and on the role of RNase Y in speB regulation.

sted, utgiver, år, opplag, sider
Taylor & Francis, 2018. Vol. 15, nr 10, s. 1336-1347
Emneord [en]
Streptococcus pyogenes, speB, RNase Y, virulence, 5' untranslated region, transcriptional regulation
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-154094DOI: 10.1080/15476286.2018.1532253ISI: 000450608900008PubMedID: 30290721OAI: oai:DiVA.org:umu-154094DiVA, id: diva2:1269882
Forskningsfinansiär
Göran Gustafsson Foundation for Research in Natural Sciences and MedicineThe Kempe FoundationsSwedish Research Council, K2013-57X-21436-04-3Tilgjengelig fra: 2018-12-11 Laget: 2018-12-11 Sist oppdatert: 2018-12-11bibliografisk kontrollert

Open Access i DiVA

fulltext(2491 kB)68 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2491 kBChecksum SHA-512
1babec42cbe5eef85ad791f1fff1ee56b2bd400783f5d2b15ce4969d4a64d7ef8c7d8a3d2a31b91f3d00a71f903862cdbc7a6505283acfc26b39e9a3c1b139f4
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Le Rhun, AnaïsCharpentier, Emmanuelle

Søk i DiVA

Av forfatter/redaktør
Le Rhun, AnaïsCharpentier, Emmanuelle
Av organisasjonen
I samme tidsskrift
RNA Biology

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 68 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 268 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf