The purpose of this article is to develop an understanding of business incubation along its temporal dimensions from a value creation perspective. We explore the temporal dimensions of business incubation by conducting a case study of six Swedish incubators with 43 semi-structured interviews of entrepreneurs and incubator coaches and managers. We show that temporality could be understood along the content and process of value creation. Such a delineation brings out the temporal tensions associated with the value creation processes in incubation. Our study adds to the current incubator literature by providing a more comprehensive explanation of its processes from a value creation perspective.