umu.sePublications
4445464748495047 of 244
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.ORCID iD: 0000-0001-5225-0595
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.ORCID iD: 0000-0003-2596-5936
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
2018 (English)In: Stem Cell Research & Therapy, E-ISSN 1757-6512, Vol. 9, article id 352Article in journal (Refereed) Published
Abstract [en]

Background: Adipose tissue is an excellent source for isolation of stem cells for treating various clinical conditions including injuries to the neuromuscular system. Many previous studies have focused on differentiating these adipose stem cells (ASCs) towards a Schwann cell-like phenotype (dASCs), which can enhance axon regeneration and reduce muscle atrophy. However, the stromal vascular fraction (SVF), from which the ASCs are derived, also exerts broad regenerative potential and might provide a faster route to clinical translation of the cell therapies for treatment of neuromuscular disorders.

Methods: The aim of this study was to establish the effects of SVF cells on the proliferation and differentiation of myoblasts using indirect co-culture experiments. A Growth Factor PCR Array was used to compare the secretomes of SVF and dASCs, and the downstream signaling pathways were investigated.

Results: SVF cells, unlike culture-expanded dASCs, expressed and secreted hepatocyte growth factor (HGF) at concentrations sufficient to enhance the proliferation of myoblasts. Pharmacological inhibitor studies revealed that the signal is mediated via ERK1/2 phosphorylation and that the effect is significantly reduced by the addition of 100 pM Norleual, a specific HGF inhibitor. When myoblasts were differentiated into multinucleated myotubes, the SVF cells reduced the expression levels of fast-type myosin heavy chain (MyHC2) suggesting an inhibition of the differentiation process.

Conclusions: In summary, this study shows the importance of HGF as a mediator of the SVF effects on myoblasts and provides further evidence for the importance of the secretome in cell therapy and regenerative medicine applications.

Place, publisher, year, edition, pages
BioMed Central, 2018. Vol. 9, article id 352
Keywords [en]
Adipose stem cells, Differentiation, HGF, Myoblasts, Myotubes, Myogenesis, Proliferation, SVF
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-155097DOI: 10.1186/s13287-018-1096-6ISI: 000454165400001PubMedID: 30572954OAI: oai:DiVA.org:umu-155097DiVA, id: diva2:1277534
Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved

Open Access in DiVA

fulltext(5662 kB)3 downloads
File information
File name FULLTEXT01.pdfFile size 5662 kBChecksum SHA-512
b34f46f9419da9e2e2f9239d278e4ceb7378449a8735be61268251439b7731f2b2ad5e51751c46f2ca85fb5543320a6b2f3c2cd286b1c37172e0a6c1e4a35b60
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

El-Habta, RoineSloniecka, MartaKingham, Paul J.

Search in DiVA

By author/editor
El-Habta, RoineSloniecka, MartaKingham, Paul J.
By organisation
Anatomy
In the same journal
Stem Cell Research & Therapy
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 3 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 6 hits
4445464748495047 of 244
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf