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An integrated transcriptome analysis in T-cell acute lymphoblastic leukemia links DNA methylation subgroups to dysregulated TAL1 and ANTP homeobox gene expression
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap.ORCID-id: 0000-0002-0759-3932
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi. Umeå universitet, Medicinska fakulteten, Institutionen för strålningsvetenskaper.
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2019 (Engelska)Ingår i: Cancer Medicine, ISSN 2045-7634, E-ISSN 2045-7634, Vol. 8, nr 1, s. 311-324Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Classification of pediatric T‐cell acute lymphoblastic leukemia (T‐ALL) patients into CIMP (CpG Island Methylator Phenotype) subgroups has the potential to improve current risk stratification. To investigate the biology behind these CIMP subgroups, diagnostic samples from Nordic pediatric T‐ALL patients were characterized by genome‐wide methylation arrays, followed by targeted exome sequencing, telomere length measurement, and RNA sequencing. The CIMP subgroups did not correlate significantly with variations in epigenetic regulators. However, the CIMP+ subgroup, associated with better prognosis, showed indicators of longer replicative history, including shorter telomere length (P = 0.015) and older epigenetic (P < 0.001) and mitotic age (P < 0.001). Moreover, the CIMP+ subgroup had significantly higher expression of ANTP homeobox oncogenes, namely TLX3, HOXA9, HOXA10, and NKX2‐1, and novel genes in T‐ALL biology including PLCB4, PLXND1, and MYO18B. The CIMP− subgroup, with worse prognosis, was associated with higher expression of TAL1 along with frequent STIL‐TAL1 fusions (2/40 in CIMP+ vs 11/24 in CIMP−), as well as stronger expression of BEX1. Altogether, our findings suggest different routes for leukemogenic transformation in the T‐ALL CIMP subgroups, indicated by different replicative histories and distinct methylomic and transcriptomic profiles. These novel findings can lead to new therapeutic strategies.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2019. Vol. 8, nr 1, s. 311-324
Nyckelord [en]
BEX1, DNA methylation, HOXA, pediatric acute lymphoblastic leukemia, TAL1
Nationell ämneskategori
Cancer och onkologi Hematologi Pediatrik Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-156637DOI: 10.1002/cam4.1917ISI: 000456858100032PubMedID: 30575306OAI: oai:DiVA.org:umu-156637DiVA, id: diva2:1290314
Forskningsfinansiär
KempestiftelsernaBarncancerfondenVästerbottens läns landstingVetenskapsrådetKnut och Alice Wallenbergs StiftelseTillgänglig från: 2019-02-20 Skapad: 2019-02-20 Senast uppdaterad: 2019-02-20Bibliografiskt granskad
Ingår i avhandling
1. DNA methylation signatures in precursor lymphoid neoplasms: with focus on clinical implications &  the biology behind
Öppna denna publikation i ny flik eller fönster >>DNA methylation signatures in precursor lymphoid neoplasms: with focus on clinical implications &  the biology behind
2019 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Precursor lymphoid neoplasms, namely acute lymphoblastic leukemias (ALL) and lymphoblastic lymphomas (LBL), are characterized by an aggressive proliferation of malignant progenitor B- or T-cells. To improve risk classification at diagnosis, better prognostic and treatment stratifying biomarkers are needed. Altered DNA methylation pattern is a hallmark of neoplastic transformation, and has been employed as a molecular prognostic and predictive marker in various cancers, including hematological malignancies. Our research group previously identified a CpG island methylator phenotype (CIMP) panel that classified pediatric T-ALL patients into prognostic subgroups.

The aim of this thesis was to evaluate distinct DNA methylation signatures in precursor lymphoid neoplasms, and to validate the prognostic value of CIMP classification in separate patient cohorts. Additionally, the biological mechanisms underlying the distinct CIMP methylation signatures in these malignancies were investigated.

The prognostic relevance of CIMP classification was validated in an independent Nordic cohort of pediatric T-ALL patients. Combination of CIMP status with minimal residual disease (MRD) status, could further dissect the high-risk MRD positive T-ALL patients into two CIMP subgroups with significantly distinct outcomes. Furthermore, CIMP classification at diagnosis was shown to predict overall survival in relapsed BCP-ALL patients. CIMP methylation signatures were also identified in T-LBL patients, indicating a broader relevance of CIMP based classification in lymphoid malignancies. Investigating the biology behind CIMP methylation signatures showed the association of CIMP status with the proliferative history of the leukemic cells. A differential transcriptomic analysis revealed a correlation of CIMP subgroups with known T-ALL drivers, as well as with novel genes in T-ALL biology. Finally, we identified distinct DNA methylation patterns and genetic aberrations in T-ALL and T-LBL that might contribute to the different clinical presentation of these two diseases. In conclusion, we validated the prognostic significance of CIMP methylation signature in precursor lymphoid malignancies and identified transcriptomic profiles that associated with the subgroups. DNA methylation is a strong candidate for further risk classification in lymphoid neoplasms and our findings can contribute to the identification of new potential targets for treatment.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2019. s. 82
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 2015
Nyckelord
Acute Lymphoblastic Leukemia, Lymphoma, T-ALL, BCP-ALL, T-LBL, DNA methylation, CIMP, Prognosis, TAL1, HOX.
Nationell ämneskategori
Cancer och onkologi Hematologi Pediatrik Biokemi och molekylärbiologi Genetik
Identifikatorer
urn:nbn:se:umu:diva-156642 (URN)978-91-7855-023-4 (ISBN)
Disputation
2019-03-15, Hörsal Betula, Norrlands universitetssjukhus, 90185, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2019-02-22 Skapad: 2019-02-20 Senast uppdaterad: 2019-03-14Bibliografiskt granskad

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