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“Molecular Activity Painting”: Switch-like, light-controlled perturbations inside living cells
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2017 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 56, no 21, p. 5916-5920Article in journal (Refereed) Published
Abstract [en]

Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging due to lateral diffusion of plasma membrane targeted molecules. Here we present “Molecular Activity Painting” (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable “painting” of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.

Place, publisher, year, edition, pages
2017. Vol. 56, no 21, p. 5916-5920
Keywords [en]
Optochemical biology, Photochemically induced dimerization, Rho GTPases, Signal transduction, Immobilization
National Category
Biochemistry and Molecular Biology Cell Biology Organic Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-157492DOI: 10.1002/anie.201611432OAI: oai:DiVA.org:umu-157492DiVA, id: diva2:1298432
Funder
EU, European Research Council, 678899Available from: 2019-03-22 Created: 2019-03-22 Last updated: 2019-03-28Bibliographically approved

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Wu, Yao-Wen

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