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“Molecular Activity Painting”: Switch-like, light-controlled perturbations inside living cells
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2017 (engelsk)Inngår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 56, nr 21, s. 5916-5920Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging due to lateral diffusion of plasma membrane targeted molecules. Here we present “Molecular Activity Painting” (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable “painting” of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.

sted, utgiver, år, opplag, sider
2017. Vol. 56, nr 21, s. 5916-5920
Emneord [en]
Optochemical biology, Photochemically induced dimerization, Rho GTPases, Signal transduction, Immobilization
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-157492DOI: 10.1002/anie.201611432OAI: oai:DiVA.org:umu-157492DiVA, id: diva2:1298432
Forskningsfinansiär
EU, European Research Council, 678899Tilgjengelig fra: 2019-03-22 Laget: 2019-03-22 Sist oppdatert: 2019-03-28bibliografisk kontrollert

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