umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi.ORCID-id: 0000-0001-9094-319X
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi. Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi. Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden.
Visa övriga samt affilieringar
2019 (Engelska)Ingår i: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 19, artikel-id 164Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.

Ort, förlag, år, upplaga, sidor
BioMed Central, 2019. Vol. 19, artikel-id 164
Nyckelord [en]
Herpes simplex, IgG, Avidity, ELISA, Primary infection, Reactivated infection
Nationell ämneskategori
Infektionsmedicin
Identifikatorer
URN: urn:nbn:se:umu:diva-157213DOI: 10.1186/s12879-019-3769-xISI: 000459030400003PubMedID: 30764767OAI: oai:DiVA.org:umu-157213DiVA, id: diva2:1298903
Tillgänglig från: 2019-03-25 Skapad: 2019-03-25 Senast uppdaterad: 2019-03-25Bibliografiskt granskad

Open Access i DiVA

fulltext(1331 kB)53 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1331 kBChecksumma SHA-512
050c4955c1c8f81148958a3a2864501d8efe2644f21f7d59274e126e9a83c951b16aa6debb0acfdc9b30f8e6750de501d918ce19e9e4a315fa8d993249253a32
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Olsson, JanJohansson, JörgenHonkala, EmmaLövheim, HugoElgh, Fredrik

Sök vidare i DiVA

Av författaren/redaktören
Olsson, JanJohansson, JörgenHonkala, EmmaLövheim, HugoElgh, Fredrik
Av organisationen
VirologiGeriatrik
I samma tidskrift
BMC Infectious Diseases
Infektionsmedicin

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 53 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 169 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf