umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Lipoprotein lipase in mouse kidney: effects of nutritional status and high-fat diet
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Umeå universitet, Medicinska fakulteten, Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM). Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Visa övriga samt affilieringar
2019 (Engelska)Ingår i: American Journal of Physiology - Renal Physiology, ISSN 1931-857X, E-ISSN 1522-1466, Vol. 316, nr 3, s. F558-F571Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Activity of lipoprotein lipase (LPL) is high in mouse kidney, but the reason is poorly understood. The aim was to characterize localization, regulation, and function of LPL in kidney of C57BL/6J mice. We found LPL mainly in proximal tubules, localized inside the tubular epithelial cells, under all conditions studied. In fed mice, some LPL, colocalized with the endothelial markers CD31 and GPIHBP1 and could be removed by perfusion with heparin. indicating a vascular location. The role of angiopoietin-like protein 4 (ANGPTL4) for nutritional modulation of LPL activity was studied in wild-type and Angptl4(-/-) mice. In Angptl4(-/-) mice, kidney LPL activity remained high in fasted animals, indicating that ANGPTL4 is involved in suppression of LPL activity on fasting, like in adipose tissue. The amount of ANGPTL4 protein in kidney was low, and the protein appeared smaller in size, compared with ANGPTL4 in heart and adipose tissue. To study the influence of obesity, mice were challenged with high-fat diet for 22 wk, and LPL was studied after an overnight fast compared with fasted mice given food for 3 h. High-fat diet caused blunting of the normal adaptation of LPL activity to feeding/fasting in adipose tissue, but in kidneys this adaptation was lost only in male mice. LPL activity increases to high levels in mouse kidney after feeding, but as no difference in uptake of chylomicron triglycerides in kidneys is found between fasted and fed states, our data confinn that LPL appears to have a minor role for lipid uptake in this organ.

Ort, förlag, år, upplaga, sidor
American Physiological Society , 2019. Vol. 316, nr 3, s. F558-F571
Nyckelord [en]
angiopoietin-like protein 4, high-fat diet, lipoprotein lipase, mouse, triglyceride uptake
Nationell ämneskategori
Fysiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-158108DOI: 10.1152/ajprenal.00474.2018ISI: 000462008700015PubMedID: 30698048OAI: oai:DiVA.org:umu-158108DiVA, id: diva2:1304524
Forskningsfinansiär
Vetenskapsrådet, 2015-02942Hjärt-Lungfonden, 2016-245-32MTillgänglig från: 2019-04-12 Skapad: 2019-04-12 Senast uppdaterad: 2019-04-12Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Nyrén, RakelMakoveichuk, ElenaMalla, SandhyaNilsson, Stefan K.Ericsson, MadeleneOlivecrona, Gunilla

Sök vidare i DiVA

Av författaren/redaktören
Nyrén, RakelMakoveichuk, ElenaMalla, SandhyaNilsson, Stefan K.Ericsson, MadeleneOlivecrona, Gunilla
Av organisationen
Fysiologisk kemiWallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)
I samma tidskrift
American Journal of Physiology - Renal Physiology
Fysiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 209 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf