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Characterisation of Phanerochaete chrysosporium mutants resistant to extractives of Bagassa guianensis and Prunus avium
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. (Université de Lorraine, INRA, IAM UMR1136, France)
2019 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

White rot fungi are characterized by their ability to decompose and mineralize all the wood components. During wood degradation, a multitude of toxic compounds are released including wood extractives. Wood extractives can show antifungal activities and rot fungi have evolved to cope with this stress. To improve our understanding of the detoxification processes and to identify target of extractives, a forward genetic strategy has been started using as a model the white rot fungus Phanerochaete chrysosporium RP-78. UV mutagenesis was done on P. chrysosporium conidia and a screening step was effected with lethal amounts of wood extractives from Bagassa guianensis and Prunus avium as well as rapamycin as experimental proof of concept. Consequently, collections of mutant were produced and their genome resequenced.Thereby, the aim of this study is to investigate if the selected candidate mutation is involved in the wood extractives resistance phenotype through this following strategy: (1) validate the mutation by resequencing, (2) clone the coding DNA sequence and (3) produce the corresponding proteins (WT and mutated). Simultaneously, the second aim of this study is to characterize phenotypically and physiologically the mutant collections following these questions: (1) in standard conditions, does the mutation affect the growth of the resistant mutants, (2) does the mutation confer an advantage concerning wood decaying either containing the original extractives or treated with selective extractives.The sequencing results have shown surprisingly the same sequence in WT, bag4 and bag31 mutants. However, a significantly higher wood chip degradation was reported for bag4 and bag31 compared to the WT together with a true phenotypical difference observed between bag4 and WT in response to B. guianensis woodchips. Finally, growth of bag4 and bag31 were shown to be not slowed down on poplar wood pieces impregnated with B. guianensis wood extractives compared to the positive and negative controls.

Ort, förlag, år, upplaga, sidor
2019.
Nyckelord [en]
Phanerochaete chrysosporium, wood extractives, mutants
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:umu:diva-161016OAI: oai:DiVA.org:umu-161016DiVA, id: diva2:1331378
Externt samarbete
Université de Lorraine, INRA, IAM UMR1136, France
Utbildningsprogram
Masterprogrammet i växt- och skogsbioteknik
Presentation
2019-06-17, KB.J3.01, Umeå, 10:00 (Engelska)
Handledare
Tillgänglig från: 2021-04-08 Skapad: 2019-06-26 Senast uppdaterad: 2021-04-08Bibliografiskt granskad

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NOEL, Delphine
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Institutionen för fysiologisk botanik
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Totalt: 69 träffar
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