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Effects of retrograde and light signalling on chloroplast biogenesis
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. (Strand Lab (Organelle-to-Nucleus Signalling Pathways))
2019 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Chloroplast biogenesis is a crucial developmental step during the transition from heterotrophic to autotrophic plant life. The greening process is controlled by retrograde and light signalling pathways which are converging at the nuclear-encoded GOLDEN2-LIKE1 and 2 transcription factors. Despite recent advances, the transcriptional regulation of GLK1 and GLK2 is not fully understood. Here, we offer new insights into the transcriptional control of the GLKs by applying a combination of gene expression analysis in transgenic Arabidopsis cell lines and Arabidopsis mutant seedlings. Furthermore, we investigated the crosstalk between GUN1-mediated retrograde signalling and the COP1-HY5 light signalling branch by testing the physiological responses of double and triple mutants under various conditions. Our results reveal that GUN1 represses GLK1 and GLK2 already before chloroplast development is initiated and that lifting this suppression is sufficient to induce PhANG expression in darkness. Thus, GUN1 plays an essential role as a suppressor of light response in the dark. Furthermore, we show that COP1 but not HY5 is a positive regulator of GLK1 and GLK2 expression in de-etiolated seedlings. Mutant studies suggested that HY5 and the GLKs promote chlorophyll biosynthesis in parallel pathways. Additionally, GUN1 and HY5 were shown to antagonistically regulate hypocotyl elongation in light-grown seedlings. Together, these findings provide novel insights into the complex interplay of light and retrograde signalling during chloroplast development and photomorphogenesis.

Ort, förlag, år, upplaga, sidor
2019. , s. 41
Nationell ämneskategori
Utvecklingsbiologi Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-161272OAI: oai:DiVA.org:umu-161272DiVA, id: diva2:1333409
Utbildningsprogram
Masterprogrammet i växt- och skogsbioteknik
Presentation
2019-06-18, 16:15
Handledare
Examinatorer
Tillgänglig från: 2021-04-08 Skapad: 2019-07-01 Senast uppdaterad: 2021-04-08Bibliografiskt granskad

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Umeå Plant Science Centre (UPSC)Institutionen för fysiologisk botanik
UtvecklingsbiologiBiokemi och molekylärbiologi

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