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Measurement of Yersinia translocon pore formation in erythrocytes
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College, London, UK.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Region Västerbotten. (Francis)
Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). (Francis)
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
Vise andre og tillknytning
2019 (engelsk)Inngår i: Pathogenic Yersinia: methods and protocols / [ed] Viveka Vadyvaloo and Matthew B. Lawrenz, New York, NY, U.S.A.: Humana Press, 2019, s. 211-229Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

Many Gram-negative pathogens produce a type III secretion system capable of intoxicating eukaryotic cells with immune-modulating effector proteins. Fundamental to this injection process is the prior secretion of two translocator proteins destined for injectisome translocon pore assembly within the host cell plasma membrane. It is through this pore that effectors are believed to travel to gain access to the host cell interior. Yersinia species especially pathogenic to humans and animals assemble this translocon pore utilizing two hydrophobic translocator proteins-YopB and YopD. Although a full molecular understanding of the biogenesis, function and regulation of this translocon pore and subsequent effector delivery into host cells remains elusive, some of what we know about these processes can be attributed to studies of bacterial infections of erythrocytes. Herein we describe the methodology of erythrocyte infections by Yersinia, and how analysis of the resultant contact-dependent hemolysis can serve as a relative measurement of YopB- and YopD-dependent translocon pore formation.

sted, utgiver, år, opplag, sider
New York, NY, U.S.A.: Humana Press, 2019. s. 211-229
Serie
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 2010
Emneord [en]
Biogenesis, Contact-dependent hemolysis, Effector recognition and intracellular delivery, Function and regulation, Host immune response, Membrane integration, Type III translocon pore complex
HSV kategori
Forskningsprogram
mikrobiologi; molekylärbiologi; infektionssjukdomar
Identifikatorer
URN: urn:nbn:se:umu:diva-161385DOI: 10.1007/978-1-4939-9541-7_15PubMedID: 31177441ISBN: 978-1-4939-9540-0 (tryckt)OAI: oai:DiVA.org:umu-161385DiVA, id: diva2:1335002
Forskningsfinansiär
Swedish Research Council, 2014-2105Swedish Research Council, 2009-5628Tilgjengelig fra: 2019-07-03 Laget: 2019-07-03 Sist oppdatert: 2019-07-03bibliografisk kontrollert

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