umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS).
Vise andre og tillknytning
2019 (engelsk)Inngår i: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 28, nr 6, s. 1635-1647Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Malaria represents a major global health issue, and the identification of new intervention targets remains an urgent priority. This search is hampered by more than one-third of the genes of malaria-causing Plasmodium parasites being uncharacterized. We report a large-scale protein interaction network in Plasmodium schizonts, generated by combining blue native-polyacrylamide electrophoresis with quantitative mass spectrometry and machine learning. This integrative approach, spanning 3 species, identifies > 20,000 putative protein interactions, organized into 600 protein clusters. We validate selected interactions, assigning functions in chromatin regulation to previously unannotated proteins and suggesting a role for an EELM2 domain-containing protein and a putative microrchidia protein as mechanistic links between AP2-domain transcription factors and epigenetic regulation. Our interactome represents a high-confidence map of the native organization of core cellular processes in Plasmodium parasites. The network reveals putative functions for uncharacterized proteins, provides mechanistic and structural insight, and uncovers potential alternative therapeutic targets.

sted, utgiver, år, opplag, sider
Elsevier, 2019. Vol. 28, nr 6, s. 1635-1647
Emneord [en]
Plasmodium, blue native-PAGE, interactome, protein-protein interactions, interaction network, malaria, Plasmodium falciparum, Plasmodium berghei, Plasmodium knowlesi
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-162665DOI: 10.1016/j.celrep.2019.07.019ISI: 000478978200023PubMedID: 31390575OAI: oai:DiVA.org:umu-162665DiVA, id: diva2:1348524
Tilgjengelig fra: 2019-09-04 Laget: 2019-09-04 Sist oppdatert: 2019-09-04bibliografisk kontrollert

Open Access i DiVA

fulltext(10170 kB)92 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 10170 kBChecksum SHA-512
4cd1d2a6615bbe7e1b550471a42769f515c65e49410cad35944e278dad79f64ad7f0cdbe41fd25fe90e7d6fc5facb9aebd0832409ee3d93bc9b7999dbef64698
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Bushell, EllenBillker, Oliver

Søk i DiVA

Av forfatter/redaktør
Bushell, EllenBillker, Oliver
Av organisasjonen
I samme tidsskrift
Cell reports

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 92 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 199 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf