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Electrochemical sensing of bacteria via secreted redox active compounds using conducting polymers
Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS).
2019 (engelsk)Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 297, artikkel-id UNSP 126703Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Bacterial infections and antibiotic resistance represent major global threats to public health. Current diagnostics use culture based assays that are reliable but slow, hence appealing for new rapid methods. Here we describe redox sensing as a novel concept for rapid, label-free detection of bacteria. We utilize a two-electrode poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) based sensor for detection of bacterially secreted redox-active compounds. Using purified redox-active compounds, we show the ability of the sensor to detect and quantify compounds in micromolar concentrations within minutes. When applied for detection and quantification of Salmonella, we show that secreted, low molecular weight redox compounds cause reduction of the PEDOT:PSS electrode. A potential role of redox sensing in infection diagnostics was demonstrated as uropathogenic strains of E. coli., Staphylococcus, Enterococcus, Pseudomonas, Proteus, and Klebsiella spp., major causes of complicated urinary tract infections, were successfully detected in complex media or processed urine. Since numerous bacterial species are capable of extracellular electron transfer, redox sensing may find use as a generic method for bacterial detection with applications in research laboatories, the clinic and industry alike.

sted, utgiver, år, opplag, sider
ELSEVIER SCIENCE SA , 2019. Vol. 297, artikkel-id UNSP 126703
Emneord [en]
Salmonella, Uropathogens, Redox, Sensor, Diagnostics, Organic bioelectronic
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-162295DOI: 10.1016/j.snb.2019.126703ISI: 000478562700038OAI: oai:DiVA.org:umu-162295DiVA, id: diva2:1349778
Tilgjengelig fra: 2019-09-10 Laget: 2019-09-10 Sist oppdatert: 2019-09-10bibliografisk kontrollert

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