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A novel molecular circuit guides IAA-JA crosstalk during adventitious rooting in Arabidopsis
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. (Catherine Bellini)
2019 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 80 poäng / 120 hpStudentuppsats (Examensarbete)
Abstract [en]

Adventitious rooting is a post embryonic developmental program controlled by a myriad of environmental and endogenous cues. Adventitious roots (ARs) usually initiate from pericycle or cambium-like cells, which undergo reprogramming and cell-fate transition programs. Phytohormones such as indole-3-acetic-acid (IAA) or Jasmonate (JA) play a prominent role in guiding these programs. Indeed, we have previously shown that IAA and JA crosstalk to control early events of adventitious root initiation (ARI) in Arabidopsis thaliana (Arabidopsis). Auxin mediates ARI by controlling the expression of GRETCHEN HAGEN3 (GH3) genes, which modulate the JA pools. JA  in turn negatively regulates ARI in a CORONATINE INSENSITIVE 1 (COI1)-dependent manner. The genetic components acting downstream of JA signalling and the exact mechanistic basis of auxin and JA crosstalk during ARI remains elusive. The present work aimed to bring more insights on the role of JA during ARI and identify its downstream targets. First we adopted  a cell-type-specific complementation approach to unravel where JA signalling inhibits ARI. We found that JA signalling possibly represses the early events occurring in the pericycle cells leading to ARI.  Next, we adopted a comparative transcriptomic approach to unravel the downstream targets of JA signalling during ARI. We identified novel genetic components (DIOXYGENASE FOR AUXIN OXIDATION DAO1 and DAO2) acting downstream of JA. DAO genes control IAA oxidation which leads to an irreversible degradation of IAA. We provide evidence that COI1-dependent JA signalling controls the expression of DAO1 and DAO2.We also show that DAO1 is the major player in ARI, as revealed by the characterization of the double mutant dao1-1dao2C. The mutant dao2C was generated using CRISPR-Cas9 technology. Furthermore, we demonstrated that the loss-of- function mutant dao1-1 exhibited an enhanced AR phenotype compared to the wild type. This phenotype was possibly due to JA and its bioactive form JA-Ile deficiency. Altogether, our data indicates that DAO1 controls a hormonal feedback circuit that maintains and stabilizes the auxin and JA crosstalk during ARI.

Ort, förlag, år, upplaga, sidor
2019. , s. 39
Nyckelord [en]
Adventitious roots, Arabidopsis, Auxin, Jasmonate
Nationell ämneskategori
Växtbioteknologi
Identifikatorer
URN: urn:nbn:se:umu:diva-163432OAI: oai:DiVA.org:umu-163432DiVA, id: diva2:1352754
Utbildningsprogram
Masterprogrammet i växt- och skogsbioteknik
Presentation
2019-09-17, KB.G4.02, Umeå, 11:15 (Engelska)
Handledare
Examinatorer
Tillgänglig från: 2021-04-08 Skapad: 2019-09-19 Senast uppdaterad: 2021-04-08Bibliografiskt granskad

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Umeå Plant Science Centre (UPSC)Institutionen för fysiologisk botanik
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