umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Evaluation of growth, stemness, and angiogenic properties of dental pulp stem cells cultured in cGMP xeno-/serum-free medium
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).ORCID iD: 0000-0002-1710-7715
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. Division of Clinical Immunology and Transfusion Medicine, Tissue Establishment, Cell Therapy Unit, Department of Laboratory Medicine, Umeå University Hospital, Umeå, Sweden.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).ORCID iD: 0000-0003-2596-5936
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
2019 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878Article in journal (Refereed) Epub ahead of print
Abstract [en]

This study was aimed to investigate the effects of cGMP xeno-/serum-free medium (XSF, Irvine Scientific) on the properties of human dental pulp stem cells (DPSCs). DPSCs, from passage 2, were cultured in XSF or fetal bovine serum (FBS)-supplemented medium, and sub-cultured up to passage 8. Cumulative population doublings (PDs) and the number of colony-forming-units (CFUs) were determined. qRT-PCR, ELISA, and in vitro assays were used to assess angiogenic capacity. Flow cytometry was used to measure CD73, CD90, and CD105 expression. Differentiation into osteo-, adipo-, and chondrogenic cell lineages was performed. DPSCs showed more elongated morphology, a reduced rate of proliferation at later passages, and lower CFU counts in XSF compared with FBS. Expression of angiogenic factors at the gene and protein levels varied in the two media and with passage number, but cells grown in XSF had more in vitro angiogenic activity. The majority of early and late passage DPSCs cultured in XSF expressed CD73 and CD90. In contrast, the percentage of CD105 positive DPSCs in XSF medium was significantly lower with increased passage whereas the majority of cells cultured in FBS were CD105 positive. Switching XSF-cultured DPSCs to medium supplemented with human serum restored the expression of CD105. The tri-lineage differentiation of DPSCs cultured under XSF and FBS conditions was similar. We showed that despite reduced CD105 expression levels, DPSCs expanded in XSF medium maintained a functional MSC phenotype. Furthermore, restoration of CD105 expression is likely to occur upon in vivo transplantation, when cells are exposed to human serum.

Place, publisher, year, edition, pages
Springer, 2019.
Keywords [en]
CD105, cGMP xenogeneic serum-free culture, Human dental pulp stem cells, Mesenchymal stromal cell culture
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-167049DOI: 10.1007/s00441-019-03160-1ISI: 000504884900001PubMedID: 31889209Scopus ID: 2-s2.0-85077198353OAI: oai:DiVA.org:umu-167049DiVA, id: diva2:1383966
Available from: 2020-01-09 Created: 2020-01-09 Last updated: 2020-01-09

Open Access in DiVA

fulltext(4162 kB)10 downloads
File information
File name FULLTEXT01.pdfFile size 4162 kBChecksum SHA-512
316f1b80a8add8d94db7055cdbd426027a35caaec2f2c1bf735d8750e18b2fb16c4c781d54cf7166e98e4f63bd396973057383c8ed8afcc106fe2624bbad59c3
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records BETA

Qu, ChengjuanBrohlin, MariaKingham, Paul J.Kelk, Peyman

Search in DiVA

By author/editor
Qu, ChengjuanBrohlin, MariaKingham, Paul J.Kelk, Peyman
By organisation
Department of Integrative Medical Biology (IMB)Department of Clinical Microbiology
In the same journal
Cell and Tissue Research
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 10 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 44 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf