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Electronic cigarettes containing nicotine increase endothelial and platelet derived extracellular vesicles in healthy volunteers
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Section of Sustainable Health.ORCID iD: 0000-0002-1630-3167
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine.
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2020 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484Article in journal (Refereed) Epub ahead of print
Abstract [en]

BACKGROUND AND AIMS: E-cigarette use is increasingly common. Whether e-cigarettes are harmful to human health is an intensely debated subject. In order to investigate whether e-cigarettes with and without nicotine cause different vascular responses, we obtained blood samples from healthy young volunteers who performed brief active e-cigarette inhalations. Extracellular vesicles (EVs) of endothelial and platelet origin were measured to determine vascular changes.

METHODS: Using a randomized, double-blind, crossover design, 17 healthy occasional smokers inhaled 30 puffs of e-cigarette vapor during 30 min. Blood samples were collected at baseline, as well as at 0, 2, 4 and 6 h post-exposure. EVs from platelets and endothelial cells were measured by flow cytometry.

RESULTS: Platelet and endothelial derived EVs were significantly increased with peak levels seen at 4 h following exposure to active inhalation of e-cigarette vapor with nicotine. Moreover, platelet derived EVs, expressing platelet activation marker P-selectin and the inflammation marker, CD40 ligand, were also significantly increased following inhalation of e-cigarette vapor with nicotine. In addition, platelet derived EVs expressing CD40 ligand was increased after inhalation of e-cigarette vapor without nicotine.

CONCLUSION: As few as 30 puffs of nicotine-containing e-cigarette vapor caused an increase in levels of circulating EVs of endothelial and platelet origin, which may signify underlying vascular changes. Although e-cigarette vapor without nicotine caused an increase in platelet EVs expressing CD40 ligand, nicotine, as a component in the vapor, seems to have a more compelling effect on extracellular vesicle formation and protein composition.

Place, publisher, year, edition, pages
Elsevier, 2020.
Keywords [en]
Electronic cigarette, Endothelial microvesicles, Extracellular vesicles, Microparticles, Platelet microvesicles, e-cigarette
National Category
Respiratory Medicine and Allergy
Identifiers
URN: urn:nbn:se:umu:diva-168670DOI: 10.1016/j.atherosclerosis.2020.02.010PubMedID: 32122618OAI: oai:DiVA.org:umu-168670DiVA, id: diva2:1412150
Available from: 2020-03-05 Created: 2020-03-05 Last updated: 2020-03-05

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Hedman, LinneaBosson, Jenny A.

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