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Low Epstein-Barr virus count in sinonasal inverted papilloma
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.ORCID-id: 0000-0003-0007-8716
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.ORCID-id: 0000-0003-3522-1842
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.ORCID-id: 0000-0002-4831-4100
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Avdelningen för virologi.ORCID-id: 0000-0001-6949-1213
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2020 (Engelska)Ingår i: Acta Oto-Laryngologica, ISSN 0001-6489, E-ISSN 1651-2251, Vol. 140, nr 5, s. 413-417Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Sinonasal inverted papilloma (SIP) is a benign tumour originating from the sinonasal mucosa showing an extensive growth pattern, a high risk of recurrence and a 5–10% risk to malignify. Epstein-Barr virus (EBV) is an oncogenic herpesvirus which infects most individuals via the saliva eliciting a latent infection. Previous studies have been reporting variable data on EBV in SIP, and there is no present appreciation regarding the association between these.

Aims/objectives: The aims were to investigate the presence and count of EBV in SIP and map the viral distribution in the epithelium versus the connective tissue.

Material and method: Fifty-three SIP patients were identified in the Pathology Department register at the University Hospital of Umeå. The biopsies were analysed with Epstein-Barr Encoded Region (EBER) in situ hybridization. EBER-positive cells were counted in the epithelium and connective tissue.

Results: We found EBER-stained cells in 30% of the cases, where 19% of these had an abundance of stained cells, and the rest showed a low count.

Conclusions/significance: These findings demonstrate a low EBV count in SIP. EBV is less likely to be a causative agent in the formation of SIP, or its malignant transformation.

Ort, förlag, år, upplaga, sidor
Taylor & Francis, 2020. Vol. 140, nr 5, s. 413-417
Nyckelord [en]
Epstein-Barr virus, sinonasal inverted papilloma, EBER-ISH
Nationell ämneskategori
Oto-rino-laryngologi
Identifikatorer
URN: urn:nbn:se:umu:diva-168828DOI: 10.1080/00016489.2020.1724330ISI: 000514736300001PubMedID: 32068495Scopus ID: 2-s2.0-85079719070OAI: oai:DiVA.org:umu-168828DiVA, id: diva2:1415575
Forskningsfinansiär
Region VästerbottenTillgänglig från: 2020-03-19 Skapad: 2020-03-19 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Ingår i avhandling
1. Mapping viruses in non-malignant tonsils, nasal polyps, sinonasal inverted papilloma and laryngeal cancer
Öppna denna publikation i ny flik eller fönster >>Mapping viruses in non-malignant tonsils, nasal polyps, sinonasal inverted papilloma and laryngeal cancer
2022 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Alternativ titel[sv]
Kartläggning av virus i godartade halsmandlar, näspolyper och inverterat papillom i näsa-bihålor samt cancer i struphuvudet
Abstract [en]

Background: The upper respiratory tract is exposed to viruses, which can lead to infection and cancer development. We chose to study common and/or chronic diseases along with common and cancer related viruses in the upper airway. High-risk human papillomavirus (HPV) causes cancer in tonsils and base of tongue, and Epstein-Barr virus (EBV) in the nasopharynx. p16 is used as a site-specific tumor marker for HPV. Human cytomegalovirus (HCMV) and human adenovirus (HAdV) are proposed to be oncomodulatory. It is unclear what significance these viruses have in benign tonsillar disease, chronic rhinosinusitis with nasal polyps (CRSwNP), sinonasal inverted papillomas (SIP) and laryngeal squamous cell carcinoma (LSCC). If virus is identified, it could make possible the use of current vaccines in prevention and treatment, as well as protection of healthcare providers.

Material and Methods: We analyzed 40 benign tonsils, 45 paired nasal polyp and healthy nasal mucosa samples, 53 SIP and 78 LSCC samples. We used PCR/microarrays (PapilloCheck®) for HPV detection and genotyping, immunohistochemistry (IHC) for p16 expression and real-time PCR for EBV, HCMV and HAdV detection. Additionally, Epstein-Barr encoding region (EBER) in situ hybridization (ISH) was used for EBV localization and count.

Results: HPV and p16 were not co-expressed, and p16 levels were low in benign tonsils, nasal polyps, and paired controls. Also, 9% of LSCC samples were high-risk HPV 16 positive and over-expressed p16.

EBV-positive cells were detected in 65% of the tonsils, nasal polyps (36%) versus controls (12%), 30% of SIP cases and 33% of LSCC samples.

Conclusions: EBV is commonly identified in benign tonsils, nasal polyps, SIP and LSCC, when using sensitive and robust detection methods. At the same time, viral infection with HPV, HCMV or HAdV appears to be uncommon in these conditions. p16 does not emerge as a reliable marker for HPV infection in LSCC.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2022. s. 72
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 2188
Nyckelord
Human papilloma virus, Epstein-Barr virus, Human cytomegalovirus, Human adenovirus, p16 tumor suppressor protein, Non-malignant tonsillar disease, Chronic rhino sinusitis with nasal polyps, sinonasal inverted papilloma, laryngeal cancer, EBER-ISH
Nationell ämneskategori
Oto-rino-laryngologi
Forskningsämne
oto-rhino-laryngologi
Identifikatorer
urn:nbn:se:umu:diva-200180 (URN)978-91-7855-841-4 (ISBN)978-91-7855-842-1 (ISBN)
Disputation
2022-11-25, Hörsalen Snäckan, Hus 16, Östersunds Sjukhus, Östersunds sjukhus, Kyrkgatan 16, Östersund, 09:00 (Svenska)
Opponent
Handledare
Tillgänglig från: 2022-11-04 Skapad: 2022-10-12 Senast uppdaterad: 2022-10-13Bibliografiskt granskad

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Schindele, AlexandraHolm, AnnaNylander, KarinAllard, AnnikaOlofsson, Katarina

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