umu.sePublications
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Apolipoprotein E Interferes with IAPP Aggregation and Protects Pericytes from IAPP-Induced Toxicity
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.ORCID iD: 0000-0001-9070-6215
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.ORCID iD: 0000-0002-3430-3101
Show others and affiliations
2020 (English)In: Biomolecules, E-ISSN 2218-273X, Vol. 10, no 1, article id 134Article in journal (Refereed) Published
Abstract [en]

Apolipoprotein E (ApoE) has become a primary focus of research after the discovery of its strong linkage to Alzheimer’s disease (AD), where the ApoE4 variant is the highest genetic risk factor for this disease. ApoE is commonly found in amyloid deposits of different origins, and its interaction with amyloid-β peptide (Aβ), the hallmark of AD, is well known. However, studies on the interaction of ApoEs with other amyloid-forming proteins are limited. Islet amyloid polypeptide (IAPP) is an amyloid-forming peptide linked to the development of type-2 diabetes and has also been shown to be involved in AD pathology and vascular dementia. Here we studied the impact of ApoE on IAPP aggregation and IAPP-induced toxicity on blood vessel pericytes. Using both in vitro and cell-based assays, we show that ApoE efficiently inhibits the amyloid formation of IAPP at highly substoichiometric ratios and that it interferes with both nucleation and elongation. We also show that ApoE protects the pericytes against IAPP-induced toxicity, however, the ApoE4 variant displays the weakest protective potential. Taken together, our results suggest that ApoE has a generic amyloid-interfering property and can be protective against amyloid-induced cytotoxicity, but there is a loss of function for the ApoE4 variant.

Place, publisher, year, edition, pages
MDPI, 2020. Vol. 10, no 1, article id 134
Keywords [en]
apolipoprotein E, IAPP amyloid, Thioflavin T, pericytes, cytotoxicity
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-168881DOI: 10.3390/biom10010134ISI: 000514863200033PubMedID: 31947546OAI: oai:DiVA.org:umu-168881DiVA, id: diva2:1415624
Funder
The Swedish Brain Foundation, FO2018-0334Swedish Research Council, 199-0469Stiftelsen Gamla Tjänarinnor, 2018-00718Available from: 2020-03-19 Created: 2020-03-19 Last updated: 2020-03-19Bibliographically approved

Open Access in DiVA

fulltext(2384 kB)10 downloads
File information
File name FULLTEXT01.pdfFile size 2384 kBChecksum SHA-512
14bd2977bfd782a985dede72745064cdbe3632d06cbcc8eef647dc02d501d6b27ecffeec4c019a70f893602be8389ba3f54cce9d6ac7e5ad0d6624db080fdb60
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Gharibyan, AnnaIslam, TohidulOlofsson, Anders

Search in DiVA

By author/editor
Gharibyan, AnnaIslam, TohidulGolchin, Solmaz A.Schultz, NinaOlofsson, Anders
By organisation
Department of Medical Biochemistry and Biophysics
In the same journal
Biomolecules
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 10 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf