umu.sePublications
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Subtle structural alterations in G-quadruplex DNA regulate site specificity of fluorescence light-up probes
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0002-7268-9519
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0001-7691-4392
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Biophysics, Molecular Biology & Bioinformatics, University of Calcutta, 92, APC Road, Kolkata 700009, India.
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0001-6347-2169
Show others and affiliations
2020 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 48, no 3, p. 1108-1119Article in journal (Refereed) Published
Abstract [en]

G-quadruplex (G4) DNA structures are linked to key biological processes and human diseases. Small molecules that target specific G4 DNA structures and signal their presence would therefore be of great value as chemical research tools with potential to further advance towards diagnostic and therapeutic developments. However, the development of these types of specific compounds remain as a great challenge. In here, we have developed a compound with ability to specifically signal a certain c-MYC G4 DNA structure through a fluorescence light-up mechanism. Despite the compound's two binding sites on the G4 DNA structure, only one of them result in the fluorescence light-up effect. This G-tetrad selectivity proved to originate from a difference in flexibility that affected the binding affinity and tilt the compound out of the planar conformation required for the fluorescence light-up mechanism. The intertwined relation between the presented factors is likely the reason for the lack of examples using rational design to develop compounds with turn-on emission that specifically target certain G4 DNA structures. However, this study shows that it is indeed possible to develop such compounds and present insights into the molecular details of specific G4 DNA recognition and signaling to advance future studies of G4 biology.

Place, publisher, year, edition, pages
Oxford University Press, 2020. Vol. 48, no 3, p. 1108-1119
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-168878DOI: 10.1093/nar/gkz1205ISI: 000515121900015PubMedID: 31912160OAI: oai:DiVA.org:umu-168878DiVA, id: diva2:1415645
Funder
Swedish Research Council, VR-NT 2017-05235The Kempe Foundations, SMK-1632Available from: 2020-03-19 Created: 2020-03-19 Last updated: 2020-03-19Bibliographically approved

Open Access in DiVA

fulltext(5417 kB)13 downloads
File information
File name FULLTEXT01.pdfFile size 5417 kBChecksum SHA-512
9808606a528a4696b30df0e4e7fa95bc733533254fbabb295975b98cfce21983ccb996fb78742dd05ff91bc85777c52af3b2eed794a63878244c0989a5151f5b
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Kumar, RajendraChand, KaramBhowmik, SudiptaDas, Rabindra NathHedenström, MattiasChorell, Erik

Search in DiVA

By author/editor
Kumar, RajendraChand, KaramBhowmik, SudiptaDas, Rabindra NathHedenström, MattiasChorell, Erik
By organisation
Department of Chemistry
In the same journal
Nucleic Acids Research
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 13 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 29 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf