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Uptake and elimination of selected PCBs in zebra fish, three-spined stickleback and arctic char after three different routes of exposure
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
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2001 (Engelska)Ingår i: Archives of Environmental Contamination and Toxicology, ISSN 0090-4341, E-ISSN 1432-0703, Vol. 40, nr 4, s. 519-530Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The uptake and elimination of 20 structurally diverse tetra- to heptachlorinated biphenyls were studied in zebrafish (Danio rerio), three-spined stickleback (Gasterosteus aculeatus), and Arctic char (Salvelinus alpinus). The polychlorinated biphenyls (PCBs) were administered to the fish through food, intraperitoneal injection of peanut oil, or intraperitoneal implantation of silicone capsules. The retention of the PCBs in fish exposed through their diet was related with the substitution patterns of the compounds. Ortho-substituted congeners with no unsubstituted meta-para positions had high biomagnification potential. PCBs with low biomagnification all had adjacent vicinal hydrogens, indicating that congeners with this feature may have been metabolically eliminated. The retention characteristics of the PCBs in the diet-exposed and the injected zebrafish were similar. The pattern of congeners in Arctic char indicates that they have a lower capacity to metabolize PCBs compared to three-spined sticklebacks and zebrafish. The levels in the fish exposed to the PCBs through a silastic implant were negatively correlated with the hydrophobicity of the congeners. Most probably congener-specific release rates of the PCBs from the implants mask their retention characteristics. It is suggested that food, mimicking the natural intake route, should be used in PCB exposure studies to validate extrapolations to natural situations.

Ort, förlag, år, upplaga, sidor
2001. Vol. 40, nr 4, s. 519-530
Nationell ämneskategori
Kemi
Identifikatorer
URN: urn:nbn:se:umu:diva-3300DOI: 10.1007/s002440010205OAI: oai:DiVA.org:umu-3300DiVA, id: diva2:141852
Tillgänglig från: 2000-05-26 Skapad: 2000-05-26 Senast uppdaterad: 2018-06-09Bibliografiskt granskad
Ingår i avhandling
1. Physico-chemical characteristics and quantitative structure-activity relationships of PCBs
Öppna denna publikation i ny flik eller fönster >>Physico-chemical characteristics and quantitative structure-activity relationships of PCBs
2000 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The polychlorinated biphenyls (PCBs) comprise a group of 209 congeners varying in the number of chlorine atoms and substitution patterns. These compounds tend to be biomagnified in foodwebs and have been shown to induce an array of effects in exposed organisms. The structural characteristics of the PCBs influence their potency as well as mechanism of action. In order to assess the biological potency of these compounds a multi-step quantitative structure-activity relationship (QSAR) procedure was used in the project described in this thesis.

The ultraviolet absorption (UV) spectra were measured for all 209 PCBs, and digitised for use as physico-chemical descriptors. Interpretations of the spectra using principal component analysis (PCA) showed the number of ortho chlorine atoms and para-para substitution patterns to be significant. Additional physico-chemical descriptors were derived from semi-empirical calculations. These included various molecular energies, the ionisation potential, electron affinity, dipole moments, and the internal barrier of rotation. The internal barrier of rotation was especially useful for describing the conformation of the PCBs on a continuous scale.

In total 52 physico-chemical descriptors were compiled and analysed by PCA for the tetra- to hepta-chlorinated congeners. The structural variation within these compounds was condensed into four principal properties derived from a PCA for use as design variables in a statistical design to select congeners representative for these homologue-groups. The 20 selected PCBs have been applied to study structure-specific biochemical responses in a number of bioassays, and to study the biomagnification of the PCBs in various fish species.

QSARs were established using partial least squares projections to latent structures (PLS) for the PCBs potency to inhibit intercellular communication, activate respiratory burst, inhibit dopamine uptake in synaptic vesicles, compete with estradiol for binding to estrogen receptors, and induce cytochrome P4501A (CYP1A) related activities. By the systematic use of the designed set of PCBs the biological potency was screened over the chemical domain of the class of compounds. Further, sub-regions of highly potent PCBs were identified for each response measured. For risk assessment of the PCBs potency to induce dioxin-like activities the predicted induction potencies (PIPs) were calculated. In addition, two sets of PCBs were presented that specifically represent congeners of environmental relevance in combination with predicted potency to induce estrogenic and CYP1A related activities.

Ort, förlag, år, upplaga, sidor
Umeå: Kemi, 2000. s. 70
Nyckelord
polychlorinated biphenyls, PCBs, physico-chemical properties, statistical design, multivariate, PCA, PLS, biomagnification, BMF, bioassay, CYP1A, SAR, QSAR, REPs, risk assessment, Chemistry, Kemi
Nationell ämneskategori
Kemi
Identifikatorer
urn:nbn:se:umu:diva-17 (URN)91-7191-838-8 (ISBN)
Disputation
2000-05-26
Opponent
Tillgänglig från: 2000-05-26 Skapad: 2000-05-26Bibliografiskt granskad

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