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The Twin Arginine Translocation System is Essential for Virulence of Yersinia pseudotuberculosis
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
2006 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 74, no 3, p. 1768-1776Article in journal (Refereed) Published
Abstract [en]

Yersinia species pathogenic to humans have been extensively characterized with respect to type III secretion and its essential role in virulence. This study concerns the twin arginine translocation (Tat) pathway utilized by gram-negative bacteria to secrete folded proteins across the bacterial inner membrane into the periplasmic compartment. We have shown that the Yersinia Tat system is functional and required for motility and contributes to acid resistance. A Yersinia pseudotuberculosis mutant strain with a disrupted Tat system (tatC) was, however, not affected in in vitro growth or more susceptible to high osmolarity, oxidative stress, or high temperature, nor was it impaired in type III secretion. Interestingly, the tatC mutant was severely attenuated via both the oral and intraperitoneal routes in the systemic mouse infection model and highly impaired in colonization of lymphoid organs like Peyer's patches and the spleen. Our work highlights that Tat secretion plays a key role in the virulence of Y. pseudotuberculosis.

Place, publisher, year, edition, pages
American Society for Microbiology , 2006. Vol. 74, no 3, p. 1768-1776
National Category
Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:umu:diva-4775DOI: 10.1128/IAI.74.3.1768-1776.2006ISI: 000235817500037PubMedID: 16495550OAI: oai:DiVA.org:umu-4775DiVA, id: diva2:144009
Available from: 2005-10-27 Created: 2005-10-27 Last updated: 2019-01-24Bibliographically approved
In thesis
1. Virulence mechanisms of pathogenic Yersinia: aspects of type III secretion and twin arginine translocation
Open this publication in new window or tab >>Virulence mechanisms of pathogenic Yersinia: aspects of type III secretion and twin arginine translocation
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The pathogenic bacteria Yersinia pestis and Y. pseudotuberculosis are related to the degree where the former is considered a subspecies of the latter, and still they cause disease of little resemblance in humans. Y. pestis is the causative agent of lethal bubonic and pneumonic plague, while Y. pseudotuberculosis manifests itself as mild gastroenteritis. An important virulence determinant for these species is their ability to secrete and inject toxins (Yop effectors) into immune cells of the infected host, in a bacterium-cell contact dependent manner. This ability depends on the extensively studied type III secretion system, a highly complex multicomponent structure resembling a needle. The induction of Yop secretion is a strictly controlled event. The two structural type III secretion components YscU and YscP are here shown to play a crucial role in this process, which is suggested to require an YscP mediated conformational change of the C-terminus of YscU. Proteolytic cleavage of YscU within this domain is further revealed to be a prerequisite for functional Yop secretion. The needle subcomponent itself, YscF, is recognised as a regulatory element that controls the induction of Yop effectors and their polarised delivery into target cells. Potentially, the needle might act as a sensor that transmits the inducing signal (i.e. target cell contact) to activate the type III secretion system. Secondly a, for Yersinia, previously unexplored system, the Twin arginine translocation (Tat) pathway, is shown to be functional and absolutely required for virulence of Y. pseudotuberculosis. A range of putative Yersinia Tat substrates were predicted in silico, which together with the Tat system itself may be interesting targets for future development of antimicrobial treatments.

Place, publisher, year, edition, pages
Umeå: Molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2005. p. 67
Keywords
Molecular biology, Yersinia pestis, Yersinia pseudotuberculosis, bacterial pathogenesis, type III secretion, twin arginine translocation, virulence mechanisms, YscU, YscP, YscF, Molekylärbiologi
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-619 (URN)91-7305-948-X (ISBN)
Public defence
2005-11-25, Lecture Hall "Major Groove", 6L, NUS, Umeå Universitet, 901 87 Umeå, 10:00
Opponent
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Available from: 2005-10-27 Created: 2005-10-27 Last updated: 2019-01-24Bibliographically approved

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Lavander, MoaBröms, Jeanette EForsberg, Åke

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