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The Twin Arginine Translocation System is Essential for Virulence of Yersinia pseudotuberculosis
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Department of Medical Countermeasures, Division of NBC Defense, Swedish Defense Research Agency, SE-901 82 Umeå, Sweden.
2006 (Engelska)Ingår i: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 74, nr 3, s. 1768-1776Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Yersinia species pathogenic to humans have been extensively characterized with respect to type III secretion and its essential role in virulence. This study concerns the twin arginine translocation (Tat) pathway utilized by gram-negative bacteria to secrete folded proteins across the bacterial inner membrane into the periplasmic compartment. We have shown that the Yersinia Tat system is functional and required for motility and contributes to acid resistance. A Yersinia pseudotuberculosis mutant strain with a disrupted Tat system (tatC) was, however, not affected in in vitro growth or more susceptible to high osmolarity, oxidative stress, or high temperature, nor was it impaired in type III secretion. Interestingly, the tatC mutant was severely attenuated via both the oral and intraperitoneal routes in the systemic mouse infection model and highly impaired in colonization of lymphoid organs like Peyer's patches and the spleen. Our work highlights that Tat secretion plays a key role in the virulence of Y. pseudotuberculosis.

Ort, förlag, år, upplaga, sidor
American Society for Microbiology , 2006. Vol. 74, nr 3, s. 1768-1776
Nationell ämneskategori
Bioinformatik och systembiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-4775DOI: 10.1128/IAI.74.3.1768-1776.2006ISI: 000235817500037PubMedID: 16495550OAI: oai:DiVA.org:umu-4775DiVA, id: diva2:144009
Tillgänglig från: 2005-10-27 Skapad: 2005-10-27 Senast uppdaterad: 2019-01-24Bibliografiskt granskad
Ingår i avhandling
1. Virulence mechanisms of pathogenic Yersinia: aspects of type III secretion and twin arginine translocation
Öppna denna publikation i ny flik eller fönster >>Virulence mechanisms of pathogenic Yersinia: aspects of type III secretion and twin arginine translocation
2005 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The pathogenic bacteria Yersinia pestis and Y. pseudotuberculosis are related to the degree where the former is considered a subspecies of the latter, and still they cause disease of little resemblance in humans. Y. pestis is the causative agent of lethal bubonic and pneumonic plague, while Y. pseudotuberculosis manifests itself as mild gastroenteritis. An important virulence determinant for these species is their ability to secrete and inject toxins (Yop effectors) into immune cells of the infected host, in a bacterium-cell contact dependent manner. This ability depends on the extensively studied type III secretion system, a highly complex multicomponent structure resembling a needle. The induction of Yop secretion is a strictly controlled event. The two structural type III secretion components YscU and YscP are here shown to play a crucial role in this process, which is suggested to require an YscP mediated conformational change of the C-terminus of YscU. Proteolytic cleavage of YscU within this domain is further revealed to be a prerequisite for functional Yop secretion. The needle subcomponent itself, YscF, is recognised as a regulatory element that controls the induction of Yop effectors and their polarised delivery into target cells. Potentially, the needle might act as a sensor that transmits the inducing signal (i.e. target cell contact) to activate the type III secretion system. Secondly a, for Yersinia, previously unexplored system, the Twin arginine translocation (Tat) pathway, is shown to be functional and absolutely required for virulence of Y. pseudotuberculosis. A range of putative Yersinia Tat substrates were predicted in silico, which together with the Tat system itself may be interesting targets for future development of antimicrobial treatments.

Ort, förlag, år, upplaga, sidor
Umeå: Molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2005. s. 67
Nyckelord
Molecular biology, Yersinia pestis, Yersinia pseudotuberculosis, bacterial pathogenesis, type III secretion, twin arginine translocation, virulence mechanisms, YscU, YscP, YscF, Molekylärbiologi
Nationell ämneskategori
Biokemi och molekylärbiologi
Forskningsämne
molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-619 (URN)91-7305-948-X (ISBN)
Disputation
2005-11-25, Lecture Hall "Major Groove", 6L, NUS, Umeå Universitet, 901 87 Umeå, 10:00
Opponent
Handledare
Tillgänglig från: 2005-10-27 Skapad: 2005-10-27 Senast uppdaterad: 2019-01-24Bibliografiskt granskad

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Lavander, MoaBröms, Jeanette EForsberg, Åke

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