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Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode
Umeå universitet, Teknisk-naturvetenskaplig fakultet, Kemi.
Umeå universitet, Teknisk-naturvetenskaplig fakultet, Kemi.
2003 (Engelska)Ingår i: Analytical and Bioanalytical Chemistry, Vol. 375, nr 3, s. 350-5Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0.1 M NaCl and a concentration of gramicidin A in the range 0-12 nM was used. Electrochemical impedance spectroscopy in the frequency range 0.1-65 kHz was employed to investigate how the defect-free monolayer responds to interactions of gramicidin A in solution.

The data was analyzed both with multivariate data analysis and classical electrochemical methods. The principal component analysis of the resulting impedance spectra gave a linear dependence on the concentration of gramicidin A. An increasing permittivity was observed in the low-frequency regime with increasing concentration of gramicidin A in solution.

Ort, förlag, år, upplaga, sidor
2003. Vol. 375, nr 3, s. 350-5
Nyckelord [en]
Phospholipid monolayer, Gramicidin A, DOPC, Impedance spectroscopy, Multivariate analysis
Identifikatorer
URN: urn:nbn:se:umu:diva-9963DOI: doi:10.1007/s00216-002-1697-9OAI: oai:DiVA.org:umu-9963DiVA, id: diva2:149634
Tillgänglig från: 2008-06-03 Skapad: 2008-06-03 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Lindholm-Sethson, Britta

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